Lab assignment 2 student copy

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University of Windsor *

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3100

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Biology

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Jan 9, 2024

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Name: Sec. No.: Medical Microbiology & Techniques Assignment for Lab #2 This assignment is based on materials posted within the “Lab Week 2: Jan 24 th - 28 th ” folder. This includes any videos shared, the lab manual, and any other materials included within the folder. This assignment is due on Monday, Jan 31 st at 11:59pm. Upload your completed assignment to Blackboard . (38 marks total) Each student must complete their own individual assignment in their own words . You can consult resources, but do not copy and paste from a website or from a friend. Note that we are using SafeAssign for most of the lab assignments. 1. What are the three different types of lab grade water and give an application of each? (5) 2. What are the two main purification methods used by the Elix-15? (2) 3. What is the function of an autoclave? (2) 4. What are three parameters of steam sterilization, and the conditions needed to achieve sterilization (3) 5. Why do you think it is necessary to pass tap water through pre-filters before it is fed to the Millipore Elix-15? (2)
6. List some safety precautions when using the autoclave (4) 7. What is a test you can run to determine the autoclave has achieved the correct conditions for sterilization? (2) 8. The display panels show the resistivity reading in MΩ cm (ELIX R in the first image and Res in the second image) for water produced from system 1 and system 2. Which system is producing the higher purity water and how do you know this? (2)
9. Why do you think you should not place an autoclave bag containing waste directly inside the autoclave without secondary containment? (2) 10. When would you would you use a vacuum cycle? (2)
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11. Give three indications this autoclave is operating correctly (3)
12. Which image shows autoclave pressure gauge readings indicate that it would be safe to open the autoclave door? (2) 13. Name the different types of biosafety cabinets, which class is recommended for working in a BSL-II Lab ? (3) #1 #2
14. The operator has commenced working in the BSC, what are they doing wrong in this picture? (1) 15. A biosafety cabinet which is being used for transferring microbial cultures. What is being done incorrectly in this picture? (1)
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16. Which type of biosafety cabinet provides protection to both the user and the environment? (2)