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The Upper extremity I
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BIO 446L
Human Microscopic & Gross Anatomy
Laboratory Manual
Spring 2023
Visible Body Team
Soroosh Sadeh, M.S.
Gonie Altman, B.S.
Supplies and Equipment:
All students must wear long pants
and closed toe shoes
in lab. Please do NOT bring FOOD OR DRINK
into lab.
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Lab 6: The Upper Extremity I
Objectives:
1. Be familiar with the brachial plexus.
2. Be familiar with bones of the upper limb and know the main features and landmarks
(clavicle, scapula and humerus).
3. Be familiar with the shoulder joint, rotator cuff muscles and its common injury.
4. Know the functions and innervation of the muscles listed in the manual (main shoulder and
elbow movers).
5. What happens to the functionality of a joint if there is a nerve damage? 6. Be familiar with joints range of motion.
7. Can you investigate potential problems with a fractured clavicle?
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Section: Brachial Plexus
Resources to use:
Visible Body Human Anatomy Atlas App.
Activity 1: Explore the Brachial Plexus and Identify the Major Nerves
Open the Human Atlas App and click on the menu button. Under nervous system views find the brachial plexus
. Click and open the view to see the innervation of the upper limb.
Brachial Plexus:
The brachial plexus is a major network of nerves supplying the anterior and posterior parts of the upper extremity musculature and skin. It begins in the lateral cervical region
(posterior triangle) and extends into the axilla. The brachial plexus consists of five sections: roots,
trunks, divisions, cords, and branches. o
Roots: Made of the anterior rami of the C5–T1 nerves
and
are essentially the spinal nerves from this region in the
spinal cord. There are 5 roots that contribute to the brachial
plexus: C5, C6, C7, C8, and T1
.
o
Trunks: There are three primary trunks made of
a combination of several of the roots (spinal nerves).
Superior (upper) trunk is a combination of the roots C5 and C6
Middle trunk is a continuation of only C7
Inferior (lower) trunk is a combination of roots C8 and T1
o
Divisions: There are two divisions;
anterior or posterior
and depend on which direction
they travel through the upper extremity.
o
Cords: There are 3 cords and they consist of
a combination of different divisions. Cords are classified by where they lie in relation to the axillary artery.
Lateral cord consists of the anterior division coming from the superior and middle trunks.
Medial cord
consists of the anterior division from the inferior trunk
Posterior cord consists of the posterior divisions from all three trunks.
o
Branches: Classified by the nerves that come off the brachial plexus. Only roots, trunks, or cords
(not divisions) can have branches. All of these branches will be distributed to the muscles and skin of the upper extremity.
Be sure to understand the makeup of the brachial plexus as show in the image below (Root, trunk, division, cord, branches).
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In the search bar, type in each of the following nerves to see their path and the muscles/regions they innervate.
o
Musculocutaneous nerve
o
Median nerve
o
Ulnar nerve
o
Radial nerve
o
Axillary nerve
o
Thoracodorsal nerve
o
Upper subscapular nerve
o
Suprascapular nerve
o
Lower subscapular nerve
These are some of the major branches. You must know them and the muscles they innervate. The muscles you need to know with their innervation will be introduced during this lab and the next lab.
Section: Shoulder and Arm Anatomy
Resources to use:
Visible Body Human Anatomy Atlas App.
Disarticulated skeleton model in the lab.
Activity 1: Bones of the Shoulder Girdle and their Landmarks
Be able to identify the bones of the shoulder girdle and the upper arm as well as their bony landmarks: clavicle
, scapula, and humerus.
Clavicle + Scapula: Make up the pectoral/shoulder girdle and are responsible for attaching the upper limbs to the skeleton and provide attachment points for the shoulder muscles.
In Human Anatomy Atlas app, i
n the Skeletal System Views, select View 16. Axillary Region to observe how muscles attach to the clavicles and scapulae.
o
You can select the systems icons on the left side of the screen to hide blood vessels, lymphatic vessels, and nerves.
The shoulder has the greatest range of motion of any joint in the body, and the many muscles that
attach here are known as stabilizer muscles due to its large range of motion (ROM).
Scapula
In the Human Anatomy Atlas app click on the scapula and isolate the bone. Find the landmarks listed below and then do the same with the models on your table.
Note: You are only responsible for knowing the landmarks that are italicized.
o
Glenoid cavity
o
Spine
o
Acromion
o
Coracoid process
o
Infraspinous fossa
o
Supraspinous fossa
o
Subscapular fossa
o
Inferior angle
o
Superior angle
o
Lateral border
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o
Medial border
o
Neck
o
Acromial angle
Describe how you would distinguish between a right and left scapula.
Clavicle
Next isolate the clavicle and find the following landmarks. Then try and find these same landmarks on the model. (you may add the image of your labeled bone here)
Note: You are only responsible for knowing the landmarks that are italicized
.
o
Acromial end
o
Sternal end
o
Shaft
o
Conoid tubercle
Describe in which direction the clavicle curves and how it articulates with other bones.
Humerus
Isolate the humerus bone and find the following landmarks. Then try and find these same landmarks on your disarticulated skeleton.
Note: You are only responsible for knowing the landmarks that are italicized
.
o
Head
o
Anatomical neck
o
Surgical neck
o
Shaft
o
Greater tubercle
o
Lesser tubercle
o
Intertubercular groove
o
Radial groove
o
Deltoid tuberosity
o
Trochlea
o
Capitulum
o
Coronoid fossa
o
Olecranon fossa
o
Medial epicondyle
o
Lateral epicondyle
o
Radial fossa
What is the difference between the anatomical neck and the surgical neck of the humerus?
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Review your answers with your group mates and your TAs.
Section: Muscles of The Shoulder (glenohumeral) Joint
Resources to use:
Visible Body Human Anatomy Atlas App
Visible Body Physiology and Anatomy App
Visible Body Muscle Premium App
Upper Limb Models in the lab
The Shoulder Joint Skeleton Model
Introduction:
The muscles in and around the shoulder girdle act on the bones to move this joint.
The glenoid cavity of the scapula is shallow and does not snugly fit the head of the humerus which causes the tendons of the muscles in this area to act as stabilizers for the humerus bone.
Movement of the brachium, or upper arm, relies on the fixators muscles of the shoulder to keep the scapula in place, which allows the arm can move freely.
The antebrachium (forearm) and hand muscles begin to get smaller and more numerous, which allows for fine motor skills.
The arm and forearm are broke down into anterior and posterior compartments
o
Anterior compartment of the arm: Flexes o
Anterior compartment of the forearm: flexion of the wrist
o
Posterior compartment of the forearm: extension of the wrist
o
Posterior compartment of the arm: Extends
Access 3D views and animated muscle actions in Visible Body’s Human Anatomy Atlas or in Muscle Premium App to visualize muscle actions. o
As a Reminder, when you select a structure in the Atlas app, you’ll see a blue pin icon in the content box. This will give you the option to view origins and insertions as visible pins on the muscle (select Attachments), view the blood supply, and/or the nerve supply.
Origin: The attachment of a muscle above the joint.
Insertion: The attachment of the muscle past a joint and is distal to the bone below
Muscles that traverse more than one joint can work at both joints; however, they cannot work at both joints at the same time.
o
Note: You won’t be asked about the muscle attachments in your quizzes or exams, but you
are strongly encouraged to pay attention to them as they help you understand the muscle functions.
Activity 1: Shoulder Anatomy and Function
Launch Muscle Premium and navigate to Pathologies, then find Rotator Cuff Tear under Shoulder.
Under Related Content, select the first video, “Shoulder Anatomy and Function”.
Watch the video and answer the questions below in 1-2 sentences:
o
The shoulder joint is formed by which two bones?
o
Describe the shoulders range of motion compared to other joints in the body.
o
Name the two categories into which the muscles of the shoulder joint can be classified.
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The Upper extremity I
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o
Name three static stabilizers for the shoulder and explain the overall function of these muscles.
o
Dynamic stabilizers are muscles that surround the joint and when they contract produce movement. A specific group of muscles under this classification are the rotator cuff muscles. They compress the head of the humerus into the glenoid fossa and stabilize movements in all directions. Name the four muscles of the rotator cuff.
Activity 2: Rotator Cuff Anatomy and Function
Using the right arrow, navigate to slide 3 of 3 entitled “Normal Anatomy.”
In the view, select each muscle and bone to learn its name. Then, identify the structures indicated by each letter on the image below, and fill in their names in the corresponding spaces beneath the image. Can you identify any of these muscles on your shoulder joint model?
A.
A.
B.
B. C. D. E. F.
Select each muscle, then select the
icon to learn their actions. List their actions, attachments and innervation in the table below.
Rotator Cuff Muscles
Muscle
Origin
Insertion
Action
Innervation
Infraspinatus
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Supraspinatus
Subscapularis
Teres minor
Activity 3: Rotator Cuff Tear
In Muscle Premium App navigate to Pathologies, then find Rotator Cuff Tear under Shoulder.
Select the
icon and read the description of rotator cuff tears. Then answer the following questions in 1-2 sentences.
o
Gradual degeneration or a sudden traumatic event can tear what structures of the rotator cuff?
o
What are the symptoms of this injury?
o
Where is the common location of rotator cuff tears?
o
Tears are usually categorized in two ways. Explain the two categories.
o
Which muscle of the rotator cuff is most commonly torn?
Activity 4: Partial Tear of the Supraspinatus
Using the right arrow, navigate to slide 2 of 3 entitled “Partially Torn Supraspinatus.”
o
Note the tear in the tendon of the supraspinatus. Describe the location of the tear and why
it’s a partial tear in 1-2 sentences.
o
Select the
icon to learn more about the partially torn supraspinatus. What is the function of the supraspinatus? o
The supraspinatus tendon can begin to fray from overuse, poor posture, or the wear and tear of aging. It can also fray due to excessive strain. Based on what you’ve read, list two examples of actions that may involve excessive strain and cause a tear.
Fill in the blanks: A traumatic event such as ______ can also cause tears of the supraspinatus tendon. Fraying and degenerative tears can also be caused by chronic ______
.
Activity 5: Arm Synergists / Fixators
Open the Visible Body Anatomy and Physiology App.
View Module 16.34 (Arm: Synergists / Fixators) and Module 16.35 (Arm: Rotator Cuff).
These muscles primarily act to stabilize the scapula and move the arm and four of them are part of the rotator cuff muscles previously mentioned.
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Complete the following table. You need to understand each muscle’s action and innervation.
Arm: Synergists / Fixators Muscle
Origin
Insertion
Action
Innervation
Coracobrachialis
Teres major
Activity 6: Prime Movers of the Arm
In Visible Body Anatomy and Physiology App, view Module 16.33 (Arm: Prime Movers)
The muscles listed in the chart below are prime movers of the arm which means they all cross the shoulder joint to insert on the humerus. Where the muscle crosses the joint will determine how the humerus moves.
Complete the following table. You need to understand each muscle’s action and innervation.
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Arm: Prime Movers
Muscle
Origin
Insertion
Action
Innervation
Pectoralis major
Deltoid
Latissimus dorsi
Review your answers with your group mates and your TAs.
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Section: Muscles of the Elbow Joint
Resources to use:
Visible Body Human Anatomy Atlas App or Visible Body Muscle Premium App
Visible Body Physiology and Anatomy App
Upper Limb Models in the lab
Activity 1: Elbow Flexors
View Module 16.36 Forearm: Elbow Flexors
These muscles are all located on the anterior side of the humerus and cross the elbow to insert on the radius or ulna (we will study these bones in the next lab). o
Biceps brachii is named for its “two heads;” note the two different origins of this muscle.
Complete the following table. You need to understand each muscle’s action and innervation.
Forearm: Elbow Flexors
Muscle
Origin
Insertion
Action
Innervation
Biceps brachii (long
and short heads)
Brachialis
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Brachioradialis
Activity 2: Elbow Extensors
View Module 16.37 (Forearm: Elbow Extensors)
These muscles are located on the posterior side of the forearm and cross the elbow joint. Note the three different origins of the three heads of the triceps brachii.
Complete the following table. You need to understand each muscle’s action and innervation.
Forearm: Elbow Extensors
Muscle
Origin
Insertion
Action
Innervation
Triceps brachii
(medial head)
Anconeus
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The Upper extremity I
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Review your answers with your group mates and your TAs.
Section: Review of the Muscles of the Shoulder and Elbow
*TA talking point.
Using the biceps muscle, we will describe its various actions at the shoulder and elbow joints. This is an example of a muscle that passes more than one joint and has multiple actions at these joints.
Major Muscles of the Shoulder (glenohumeral) joint
o
Flexion:
Anterior Deltoid, Biceps, Coracobrachialis, Pectoralis major (Clavicular head).
o
Extension:
Posterior Deltoid, Triceps (long head), Latissimus dorsi (from the flexed position), Teres major.
o
Abduction:
Lateral Deltoid, Supraspinatus.
o
Adduction:
Pectoralis major, Teres major, Latissimus dorsi, coracobrachialis.
o
Medial rotation:
Pectoralis major, Latissimus dorsi, Teres major, Subscapularis, anterior
deltoid.
o
Lateral rotation:
Infraspinatus, Deltoid (spiral part), Teres minor.
Focus on the muscles listed above and disregard the minor muscles you may see while working on the modules. You need to be able to identify the muscles on the models in the lab. **Remember, that sometimes a joint must be placed in hyperextension or flexion for that muscle to work as described. Activity 1: Review Questions/Nerve Damage
Which muscles are part of the rotator cuff muscles and what is their main function as a whole?
Which muscles are used when performing the following actions?
o
Raising your hand high over your head during class
o
Rowing a boat
o
Reaching behind you, arm extended and pronated
o
Reaching in front of you, arm extended and supinated
o
Bringing your hand to your heart
What would happen if the musculocutaneous nerve is cut off of the brachial plexus? o
Is there another muscle(s) with a different innervation that can still allow for flexion? YES or NO o
Can the same be said for the other actions at the shoulder? Note, this is very important in physical therapy
What muscles can flex at the elbow? Biceps Y / N, Brachialis Y / N, Pronator teres (look it up in
Human Anatomy Atlas) Y / N, Brachioradialis Y / N
o
Would you need the entire biceps muscle fibers to flex at the elbow? Y / N
o
Would you need the entire biceps muscle to flex at the elbow with a 10 lb weight in your hand? Y / N / maybe
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o
What muscle might you use to help if you cannot? Brachialis Y / N
o
Now, I am trying to pick up a 50 lb weight and flex at the elbow. Other than the biceps and brachialis muscles, what other muscles might I recruit to help? Pronator teres Y / N, brachioradialis Y / N
Because the pronator teres and the brachioradialis do other actions at the elbow, if you want to recruit them, you cannot ask them to do their other actions at the same time.
What are their other actions?
Now, what happens with flexion if you cut the musculocutaneous nerve? o
You have lost the use of what muscles?
Can you still flex at the elbow? Y / N, if yes, why? o
What is the innervation to the other muscles?
Review your answers with your group mates and your TAs.
Section: Joint Range of Motion (ROM)
Health care professionals commonly test different joints in the body for their range of motion (ROM)
. A joint’s range of motion is usually measured by the number of degrees from the starting
position of a body segment to its position at the end of its full range of motion. The starting position is the zero position (or zero degrees) which is in the anatomical position.
We use a double-armed goniometer to measure ROM, which has a stationary body segment and a moveable arm that moves along a moveable body segment. The axis of the goniometer, also known as a pin, is placed over the joint and the armed follow the movement of the two bones that create the joint. o
When anatomical landmarks are well defined, the accuracy of measurement is greater. If there is more soft tissue surrounding the joint area, measurement error can be more frequent.
Activity: Upper limb ROM
For this exercise, we will use a goniometer to make measurements. Get the TAs to assist you with the use of the goniometer.
Shoulder abduction
o
Using figure 1 below, stand behind a subject and palpate the scapula
o
Have the subject slowly abduct his/her arm at the shoulder joint.
o
Record the number of degrees of shoulder joint abduction at the point the scapula starts moving. _____
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o
Record how many degrees the scapula will move if the arm continues in abduction. ______
o
What is the name of this scapula movement?
o
What muscle(s) allows for this scapular rotation? Figure 1.
Scapula Movements
Flexion of the arm
o
Now have the subject slowly flex their arm at the shoulder joint to 90
o
o
Record the number of degrees of shoulder joint flexion at the point the scapula starts moving. _______
o
In this same position, how many centimeters does the scapula (inferior edge) move laterally, as seen in figure 2 below.
o
What is the name of this scapula movement?
Figure 2.
Lateral Motion of the Scapula (Adapted from Luttgens & Hamilton, 1997)
o
Using figure 3 below as a reference, keep the shoulder joint abducted to 90
o,
and the elbow flexed, determine and record the ranges of internal (medial) rotation ____________and external (lateral) rotation _______________.
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Figure 3. Medial and Lateral Rotation of the Shoulder
Joint or segment
Movement
Average ROM (multiple sources)
Forearm
Pronation
80-90
Supination
80-90
Shoulder
Flexion
170-180
Hyperextension
30, 50, 60, 80, depending on source
Abduction
170-180
Adduction
50
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Section: Case Study
A 15-year-old male comes to the emergency department complaining of severe pain over his right
collarbone. He uses his left hand to hold his right arm close to and across the front of his body. He explains while playing baseball, he collides with the pitcher when running to first base. The impact knocked him off his feet and he fell on his right shoulder. His mother, and others nearby, reported hearing a loud snap. When he stood up, he was slouched and complained of extreme pain on the impact area.
Radiography of his right shoulder revealed a _______ fracture. See figure below:
Figure 1
Physical Examination
The following findings were noted on physical examination:
Prominence, ecchymosis
1
, and tenderness over the right midclavicular region
Diffuse swelling over the right clavicle, shoulder, and arm
Right upper limb depressed (i.e., lower than left)
Mild numbness over right upper limb
Right radial pulse weaker than left
Lung sounds normal
Which muscles act on the clavicle? You can use Visible Body Human Atlas and find the clavicle. Click on the bone, then click on the details icon, then muscle attachment to find these muscles.
With logical reasoning and help from Human Anatomy Atlas app explain the position of shoulder and
arm (motor deficit) and clavicle due to the type and location of the injury (think of the muscles you listed in the previous question and how they are oriented relative to the clavicle and the glenohumeral
joint).
1
Ecchymosis Hemorrhage into skin (bruising)
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Some other symptoms may include:
Sensory Deficit
■ Localized pain over clavicle (commonly in the middle ¹∕³), exacerbated by arm movement
Other Deficits
■ Localized swelling and ecchymosis over clavicle
■ Skin may be “tented” or penetrated by fracture
■ Possible decreased peripheral perfusion, and/or brachial plexus injury, pneumothorax
2
, hemothorax
3
Final Clinical note
■ The proximity of the subclavian vessels, brachial plexus, and cervical pleura to the fractured clavicle makes it essential to assess the upper limb for vascular perfusion, sensation, muscle strength, as well as breath (lung exam) sounds to rule out pneumothorax and/or hemothorax. End of Lab 5, Great Job! 😊
2
Pneumothorax Air or gas in pleural cavity
3
Hemothorax Blood in pleural cavity
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