Copy of FA 23 BIO 1131 Lab 2 Worksheet

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Dec 6, 2023

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Name: BIO 1131 Fall 2023 Lab 2 Assignment Make a copy of this document, DO NOT REQUEST ACCESS. fill in your answers during and after your lab in google docs, and then download as a .docx or .pdf file to submit to Canvas by your lab section’s due date Mendelian Genetics: 1. What is the expected ratio of dominant and recessive phenotypes in a monohybrid cross? In a dihybrid cross? 3:1 ratio of phenotypes and a 9:3:3:1 2. What is the expected ratio of genotypes in a monohybrid cross? 1:2:1 Ratio 3. If a pea plant from a true-breeding strain with yellow seeds (YY) is crossed with a pea plant from a true-breeding strain with green seeds (yy), label the Punnett square below with the parental genotypes and the offspring genotypes. [Y] [Y] [y] Yy Yy [y] Yy Yy 4. For the cross above, what % of offspring will have green seeds? There's a zero percent chance of having green seeds. 5. What would happen if you took one of the offspring from the cross in #3 and self-fertilized it to produce a second generation? Draw the resulting Punnett square. [Y] [y] [Y] YY Yy [y] Yy yy 6. For the cross above, what % of offspring will have green seeds? There's a 25% chance of having green seeds.
Experiment 1: Corn Genetics 7. For this experiment, our parental generation was a cross between a plant with purple smooth kernels (RRNN) and a plant with yellow shrunken kernels (rrnn). What is the genotype of every offspring produced by this cross (the F 1 generation)? RrNn 8. The F 1 generation was self-fertilized to create the F 2 generation, and how would you represent this with a Punnett square? Fill in the table below with the parental genotypes and the offspring genotypes. [RN] [rN] [Rn] [rn] [RN] RRNN RrNN RRNn RrNn [rN] RrNN rrNN RrNn rrNn [Rn] RRNn RrNn RRnn Rrnn [rn] RrNn rrNn Rrnn rrnn Chi-Square Analysis 9. Fill out the table below using Table 4 from the lab manual. Phenotype Observed (o) Expected (e) (o e) (o e) 2 / e Purple Smooth 2,545 2419 126 6.56 Purple Shrunken 661 806 -145 26.09 Yellow Smooth 831 806 25 0.78 Yellow Shrunken 265 268 -3 .03 Total =4302 4299 3 Sum ( χ 2) =33.42 What is the chi-square value ( χ 2) ___33.46____ Degrees of Freedom = __3____
10. Look at Table 3 in your lab manual. Using your DF, determine the critical value that you will use to evaluate your chi-square results. Your chi-square value: _____33.46________ Critical chi-square value: ______7.81________ 11. Is your value less than or greater than the critical chi-square value? My values is greater than the critical chi square value. 12. Do your results allow you to reject or accept the null hypothesis? reject 13. Did your class’ results for this experiment match your expectations for Mendelian inheritance? Why or why not? yes it did match somewhat of my expectations. Reason being because corn is genetically modified. So i Knew the results would be all over the place.
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