Pre-lab (Week 3)

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Skyline College *

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253L

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Biology

Date

Dec 6, 2023

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docx

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2

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Pre-lab questions for Biol 253L, week 3. The answers are found in this week’s reading in the Lab Manual. Answer in the space below and continue on back if necessary. Uploaded onto Canvas once completed. 1. What does the name "BLAST" stand for? BLAST stands for Basic Local Alignment Search Tool. 2. What is the purpose of doing a BLAST search? Blast was designed to search nucleotide and protein databases. 3. When you get your BLAST results, based on what criteria are the alignments (high- scoring subject pairs) ranked? High-scoring subject pairs (HSPs) are ranked based on length and significance. 4. Will you be doing a BLAST search with your gene’s nucleotide sequence? If no, then which sequence will you be using? No, we will not be using BLAST search because will be using protein sequence. 5. Why is it better to use BLASTp than BLASTn to identify homologs? BLASTp has a higher probability in indentifying a homolog compared to BLASTn. BLASTp's capabilities include the ability to locate potential domains in the query protein by matching its segments to its closest neighbors. 6. What is the definition of an "E-value"? If an E-value is very high, what does this mean? “E-value” describes the probability that the observed alignments are the result of chance due to randomized alignments. The higher the E-value, the difficulty it may be to locate a sequence by chance. 7. When two sequences share a common ancestor, those sequences are said to be Homology . 8. What is your objective in performing dihybrid crosses with Fast Plants during Experiment 2-2? The objective in performing dihybrid crosses with Fast Plants is to focus on what two genes are responsible for plant color in B. rapa.
9. How and when will you pollinate your plants? When flowers start to show, we will be delivering the pollen grains from P1 plant to the stigma of a flower on P2 plant. To pollinate our plants, we will be using a chenille rod. Using the rod to gently touch the anthers of one flower, we are going to collect the yellow pollen grains. We will be doing this for at least 4 flowers on each plant.
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