LAB 6 - Cell Structure & Microscope Review(2)

Instructions: Strictly, use any word or a 2–3-word phrase to describe and distinguish each term. Do not  repeat or re-mention the term asked on your answers. Present your answers in a table.  (per term must be described, so per item/number, 2 terms are described) endoplasmic reticulum and ribosome  amyloplast and chromoplast  Golgi apparatus and dictyosome  proplastids and etioplasts  thylakoid and grana  desmotubule and plasmodesmata

Name Date Cell Structure Lab Report Directions: Complete each lab procedure in the order it is presented. Be sure to document your investigation by thoroughly completing each activity, such as filling in the tables, uploading any necessary photos or drawings, and answering each question. Cell Structure Gizmo Begin by reviewing the function of various organelles in plant and animal cells. You will record your results in the following table. Organelle Function Cell membrane Cell wall Centrioles Chloroplast Cytoplasm Endoplasmic reticulum Golgi apparatus Lysosomes Mitochondrion Nuclear membrane Nucleolus Nucleus Plastids Ribosomes Vacuole Vesicles © 2018 Pearson Online & Blended Learning K-12 USA. All rights reserved.

E. coli ~1X 2 micron

Is this cell an animal or plant cell? In what type of environment is this cell found? Is there a net movement of water into or out of the cell? What term is used to describe this condition? Explain your answer. Edit View Insert Format Tools Table 12pt v Paragraph v |BIU A v OT A stv MacBook Air

(ChooseI | Choose Where the slide sits on Holds the slide in place Used for making small adjustments to focus Condenses light before it goes through the sample The whole microscope sits upon this Used for moving around the stage and slide Used for making large adjustments to focus Does most of the magnification. These are close to the slide Provides light Magnifies the image, It is what you put your cycs near (Choose Choose

Using a Light Microscope to Determine an Object's SIZE PRE-LAB QUESTIONS Fill in the diagram of the microscope with the term or description that matches, the microscope part. Eye Plece Body Tube Contains lens to increase magnification usually 10x Revolves to allow changing various objectives Arm Objectives Moves stage up and down approximately to correct distance Hold slides in place Stage Permits finer focusing by moving the stage in smaller increments Regulates the amount of light going through the stage Base Light Source Copyright © 2012 Laying the Foundation®, Ic., Dallas, Texas. All rights reserved. Visit us online at www.lftralning.org.

districtims.seattleschools.org/common-assessment-delivery/start/5362404321?action3Donres Biomolecules, Cell Theory, and Microscopes HW Quiz Which microscope should you use for examining a whole, multicellular organism? Compound microscope O Stereoscopic dissecting microscope

SBI 3C1 VIRTUAL LAB: THE MICROSCOPE INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the instructions on how to properly use a microscope. When you are complete, answer the questions below. PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen? TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification) Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE ОBJECTIVE LENS POWER OCULAR MAGNIFICATION OBJECTIVE LENS MAGNIFICATION TOTAL MAGNIFICATION MAG (X) = Ocular X Objective LOW LP MAG = MEDIUM MP MAG= HIGH HP MAG- Complete the following chart by calculating the missing lens or total magnification [2] TOTAL MAGNIFICATION OBJECTIVE LENS MAGNIR AR (EYEPIECE) MAGNIFICATION 5X 80X 10X 40X 10X 100X 500X 50X PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES Access the Virtual Microscope at…

Cell Homeostasis Virtual Lab Place beaker "A" on the lab table and fill it with 1000 mL water from the graduated cylinder. RED! APODA А B C 0.00. E Please click here or on the image above to go to and start the the online lab exercise titled, "Cell Homeostasis Virtual Lab"

Instructions: Strictly, use any word or a 2–3-word phrase to describe and distinguish each term. Do not  repeat or re-mention the term asked on your answers. Present your answers in a table.  (per term must be described, so per item/number, 2 terms are described)  cellulose and cell wall  chloroplast and mitochondrium  microtubule and microfilament  cytoskeleton and microtubules

Which of the following microscopy techniques can be used in live cell imaging in a live animal. 1. Structured illumination microscopy 2. Stimulated emission depletion microscopy 3. Photoactivated localization microscopy 4. Two-photon excitation microscopy 5. Total internal reflection microscopy 6. Light-sheet microscopy O4 and 6 O2, 3 and 6 O1 and 4 3 and 5 O2 and 4

Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.

M. luteus ~0.8 micron Arrangement in tetrad (arrows) and irregular cluster 10 um

Question Completion Status: A Moving to another question will save this response. Question 13 Total Magnification of a microscope is O 1. Product of Ocular Lens and objective lens magnification O 2. Sum of the ocular lens and objective lens magnification O 3 Magnification of ocular lens minus magnification of objective lens O 4. None of above Moving to another question will save this response.

Using the provided materials and the microscope itself, review the parts of the microscope including the name, where it is located, its function and the appropriate times to use. Record your observations and notes as vou will require this information when writing yourlab report

Answer True or False Dr. Soler would like to view the internal structure of the bacteria he was working on. He should use the transmission electron microscope

Please answer the following questions in a summarized way. Also, Add bullet points. Thanks 1. Organelle that is affected by treacher Collins Syndrome and normal job of that organelle. 2. Interation of the organelle with other organelles in a normal cell.

Click and drag the appropriate tiles to label the indicated parts of a cell membrane (blue dots).  Click and drag the appropriate tiles to the yellow dots to indicate which side of the membrane represents the outside surface of the cell and which side represents the inside of the cell (cytoplasm).

districtIms.seattleschools.org/common-assessment-delivery/start/5362404321?action3Dd Biomolecules, Cell Theory, and Microscopes HW Q Which microscope should you use for examining a slide of an organism? Compound microscope O Stereoscopic dissecting microscope

Answer the following questions: Why are most cells so small? Why cell size is limited? List two instances when the coarse adjustment knob is never used Why is immersion oil used with the 100X objective? List two common problems associated with using the microscope and how you would  go about solving it. When should the lenses be cleaned?  What is the correct way to clean them?

Please include your reference below for my further research. Thank you! 1. What are the basic components of a Fluorescence Microscope and what are the functions of each?   2. Are there any parts that you can remove without compromising accuracy and utility of the equipment?  3. Can you suggest additional components to improve the equipment?

The third micrograph is at the lowest magnification. Identify A (name of the cells; blank 1). Identify B (name of the structures arrows point to; blank 2) - Identify C (name of the structures arrows point to; blank 3) High mag low mag high mag Blank # 1 Blank # 2 Blank # 3

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…

v Question Completion Status: Moving to another question will save this response. Question 10 What happens when a round cell expands in diameter? O 1. Its surface area and diameter increase with the cube of its diameter 2. Its surface area and diameter increase with the square of its diameter 3. Its volume increases faster than its surface area does O 4. Its surface area increases faster than its volume does 5. Its volume and surface area expand with the same proportion A Moving to another question will save this response. AR

Using the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 3: PLASMOLYSIS Materials: safety goggles, red onion, dropper, slides & cover slips, tweezers/ forceps, compound microscope, iodine, small knife, water, salt (5% and 10% solution) Methodology: 1. With goggles on, carefully cut the onion into wedge shaped pieces using a knife. 2. Use an eye dropper to place a drop of water in the center of a microscope slide. Use the tweezers to peel a thin layer of skin tissue from the thick part of the onion wedge and place it in the center of the microscope slide. 3. 4. 5. Add a drop of water and a drop of iodine over the onion tissue on the slide. Carefully lower a cover glass slip at an angle on the stained tissue to allow air bubbles to escape. 6. Examine the prepared slide under the compound microscope at 100X magnification. 7. Record what the cells look like. 8. Prepare a 5% salt solution by adding 5 grams of salt (measure with balance) per 100…

The following fluorescence microscopy shows several cells. What part of the cell is labelled by the fluorescence here? b The Cytosol The Endoplasmic Reticulum The nucleolus The nucleus The actin cytoskeleton

total Magnification is obtained by * Magnifying power of condenser lens Magnifying power of both the objective lens and eyepiece Magnifying power of the objective lens Magnifying power of eyepiece Which cell organelle is involved in apoptosis ER Mitochondria Golgi Lysosome