lab 1 report microscopy

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National University College *

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203A

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Biology

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Feb 20, 2024

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1 Bio 203A National university Lab 1 report: Microscopy  To use a minimum of light, which part of the microscope should be adjusted? - Iris diaphragm.  only a slight adjustment should be required. when you brought image into focus with high dry objective lens. why? - because the microscope is parfocal.  More light is usually needed. how can you increase the amount of light? - a knob that is turned clockwise in order to increase the light intensity. - the diaphragm can open & close allowing more or less light to enter the scope illuminating the object being magnified. 1. The name of the organisms observed and their taxonomical groups are as follows: Paramecium(protozoa), Spirogyra(algae), Penicillium(fungi) and Bacillus shaped bacteria (gram stain). 2. The total of magnification for all of the drawing made above were at total magnification of 40x (4x objective lens and 10x ocular lens), 400x (40x objective lens and 10x ocular lens) and 1000x (100x objective lens and 10x ocular lens). 3. the microorganism pictures below are taken under 40x objective lens.

2 Answer these questions: 1. Which objective is closest to the slide when it is in focus? - The 100x objective focuses closest to the slide when it is focus. There are the oil immersion lens. 2. Write down the correct procedure to put the microscope away. - Turn light all the way deem then turn the light switch off if available, allow microscope to sit for five minutes, wipe off the stage and take out the slide, turn stage all the way down, return objective to low magnification(4x), clean with the lens paper the lenses of

3 microscope, carry the microscope with two hands, do not twist the cable around the stage and store the microscope with its arm pointing outside. 3. You are observing samples of Bacillus cells, which are 2 micrometers long, and yeast cells, which are 10 micrometers long, you are using the high-dry(40x) lens with a FN=22mm. a) What will be the FOV for this lens and magnification? - For a high-dry (40×) lens with an FN of 22 mm, the FOV can be calculated using the following formula: FOV = (diameter of field diaphragm/magnification) x 1000 Assuming a field diaphragm diameter of 22 mm, FOV= (FN/M) *1000 FOV = (22/40) *1000 the FOV would be: 550 µm. b) How many Bacillus cells can you fit across the FOV? - The length of each Bacillus cell is given as 2 micrometers. to calculate the number of cells that can fit across the FOV, I divide the FOV by the length of each cell: - Number of Bacillus cells = FOV/ length of each Bacillus cell - Number of Bacillus cells= 550 µm/2 µm - Number of Bacillus cells = 275 cells. c) How many yeast cells can you fit across the FOV? - The length of each yeast cell is given as 10 µm. to calculate the number of cells that can fit across the FOV, I divide the FOV by the length of each cell: - number of yeast cells = FOV / length of each yeast cell - number of yeast cells = 550 µm/ 10 µm - Number of yeast cells = 55 cells.

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