LS7A Problem Solving Session 6 (Week 7)v3_Online_F20

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Feb 20, 2024

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LS7A Problem Solving Session 6 (Week 7) 1. Transcribe and translate the eukaryotic DNA sequence below. 2. Label the template/non-template strands, primary RNA, mature RNA, and the primary peptide sequences. 3. Draw in where transcription factors, RNA polymerase, and ribosomes would bind. 4. Describe the process of transcription and translation to your LA or neighbor using the terms above. 5. How many codons are in the open reading frame of the mRNA sequence? 6. How many amino acids are in the peptide sequence? 7. How many nucleotides are in the 3’UTR and 5’UTR? 8. How many polar amino acids are in this peptide sequence? Using the protein gel below choose the appropriate lane (A-E) that would result from the condition given in each statement when compared to the reference sample lane (REF) that does not contain the stated condition. 9. 60 base pair deletion in first exon. Reasoning: 10. 60 base pair insertion in first exon. Reasoning: 11. Missense mutation in exon 1. Reasoning: 12. Missense mutation in the stop codon. Reasoning: 13. Missense mutation in SRP signal sequence. Reasoning:
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