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University of Texas, San Antonio *

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1061

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Biology

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Feb 20, 2024

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éme:%ébfilfl/ vate 4-6-23 labSectionn O/ | streak Plate Methods of Isolation Experiment 1-5 Data Sheet 1. Did you achieve isolation using the quadrant streak? If so, in which streak (1, 2, 3, or 4) did it occur? If you did not achieve isolation, what might you do differently next time to improve your results? z e e i e ] 2. Using your own words, describe one method you could use to achieve isolation if you found that your plate contgins a mixed culture. 3. Answer this question if you achieve isolation using the quadrant streak. Skip this question if you did not. In the circle below, draw what you observed on the plate for this experiment. Notate where an isolated colony of each organism type are located in your drawing and describe a physical difference that you used to determine that each colony is different (i.e. do the colonies produce different pigments?) 1 f, Colony Morphology Experiment 2-2 Data Sheet 1. Usingthe terms provided in the lecture, provide the seven colony morphology characteristics for each of the organisms provided. Provide a drawing/sketch for a single colony of each organism from above (to show the shape/margin) and from the side (to show the elevation). Then provide the incubation parameters that were used for each plate observed. Scanned with CamScanner
Drawing/Sketch (whole colony) Drawing/Sketch Incubation (elevation) Parameters 2. To determine colony morphoog! 5 organism, what streak method would you use and why? KL Z 7l 2 LS L2 7o Bt Z =72, Z cbalier ol oicrecle ooy 7 ailel, Fh Chik 2 4 S=rcvn (//4"370/4 P wh N (T2, A4 1z, . 222 & )= 1" I{_ L2 l >~ 3. Out of the seven characteristics discussed, three of them tell us a lot about the organism: size, shape and color. However, physical characteristics are hot the ohly factors that are important when growing otganists. What are some other factors that can influence bacterial growth and why do you think each is crucial in the growth? W ks on 'llJ/ v, Lee £,/ 24 ; 5 Lo Fel D 4oL i s s ot Loy ot s Con Ot FE = /A’. 7 2 Blood Agar Experiment 5-21 Data Sheet Scanned with CamScanner
/ 1. :Jrsgira\fi ltsI: terms provided vln the lecture, provide the seven colony morphology characteristics for each of the A t’s provided. Det.ermlne what the hemolytic reaction for each organism Is and then provide the observation, interpretation and conclusion for each organism. (these should be provided word-for-word from the table provided in the lecture) Organism Name Colony Description ot hod used for this type of testing is usually performed from throat swab samples. Using 2. The streak and stab met| your own words, describe why we perform a streak with 3 stab into the agar. » e is typically a throat swab, why do you think a four-quadrant streak method 3. Considering that the specimen typ: ji -zaz inoculation with a st ? is performed jnstead of a simple zjg Scanned with CamScanner
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