Bacterial Growth and Inhibition POST LAB (1)

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University of North Georgia, Dahlonega *

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1101L

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Biology

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Feb 20, 2024

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BACTERIAL GROWTH AND INHIBITION – POST LAB Procedures adapted from: Microbiology Experiments, 4 th edition. J. Kleyn and M. Bicknell. McGraw-Hill, 2004. Microbiological Applications , 7 th edition. H.J. Benson. McGraw-Hill, 1998. PART 1 AND 2 Examine your plate. DO NOT OPEN THE PLATES. It is possible that some environmental samples contained pathogens (disease-causing microbes) which are now present in very high numbers. Also, mold spores can escape from open Petri dishes and contaminate an entire lab. When observing your plate, you should see mounds of microorganism cells called colonies growing on the surface of the medium. Some colonies are bacteria; they will generally have a soft appearance and can be dull or shiny. Although most bacterial colonies will be white or cream-colored, it is not unusual to see yellow or orange colonies. Colonies that are relatively large and filamentous (fuzzy) are typical of fungi. Record your observations of bacterial and fungal colonies in Table 1 (manipulate the table to fit your data). Include the following criteria: – Relative size (compared to other colonies) – Shape (round or irregular) – Color – Surface (shiny or dull) – Consistency (dry, moist, or mucoid) – Elevation (flat, craterlike, or conical) Count the total number of colonies in each half, and record your results in Table 2. Table 1 - Independent Variable Qualitative Colony Descriptions INCLUDE PICTURES OF YOUR PLATE HERE:

Table 2 - . Independent Variable Qualitative Colony Descriptions QUESTIONS 1. What was your null hypotheses? 2. What was your alternate hypothesis? 3. Which one of your hypotheses was tentatively supported and which was refuted (or not supported)? 4. In your own words, did your cleaning method work or not work? Why do you think this is? 5. Comparing the different experiments conducted in the lab, which type of environment had the greatest number of bacterial colonies? Why do you think this is? PART 3 Follow your instructor’s directions for obtaining access to your cultures. Do not open the Petri dishes. Measure the diameter of the clear area surrounding the drug disk in millimeters (mm). This clear area is called the zone of inhibition . Record your data in the table below. If a disk does not have a clear area surrounding it, the diameter of the zone is recorded as zero. Use the drug chart to determine the name of the drug you used (if you don’t already know it) and record it in the corresponding table. Use the drug chart to determine whether Escherichia coli is resistant or susceptible to the drug you tested. Record the expected result and your actual result in the table below. Obtain results from your classmates to fill in the rest of the table. Examine the zone of inhibition again very carefully. Note whether you see any colonies (individual mounds of cells) growing inside the zone of inhibition. If so, this means the cells in the colony are resistant to the drug. Dispose of plates in the designated container. Wash your hands thoroughly with soap and warm water before leaving the lab. Antibiotic Test Results Drug name Diameter of zone of inhibition for _____________________ Diameter of zone of inhibition for ___________________________

INCLUDE PICTURES OF YOUR PLATE HERE: QUESTIONS: 1. Did you notice any resistant colonies? If so, for which drugs? 2. What do you think would happen if you took resistant colonies and subcultured them (transferred them to a fresh agar plate) and exposed them to the same drug again? 3. What questions/variables would you like to test in the future?

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