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1 Acid Fast Stain-Microbiology Student’s name Institution affiliation Course Date

2 Acid Fast Stain-Microbiology Define Acid Fast Staining Acid-fast staining is a laboratory test to determine the presence of tuberculosis-causing bacteria or other illnesses. This laboratory test is usually carried out on sample blood cells, sample tissues, and other body substances like stool and sputum (Aryal, 2022). The test is performed systematically by closely analyzing these body substances on a heated, stained glass slide to flag out bacteria holding onto the first dye, considered acid-fast. The purpose of acid-fast staining The purpose is to ascertain whether or not the samples provided are infected with bacteria that cause TB. As already identified, this is a test, such that through it, a physician can disclose whether one is infected or not to lend effective medication and prescription effectively. The process of acid-fast staining Acid-fast staining is done in a laboratory. Here, a health care provider collects one of the samples of tissues, bone marrow, sputum, stool, or urine. After collecting a sample, the care provider sends it to a respective laboratory, where it is placed in a stained and heated glass slide ( Xiong et al., 2018) . Here, the cells on the sample hold onto the die, such that when the glass is washed in an acidic solution, and a different stain applied, bacteria can hold on to the first dye, a process termed acid-fast, for its tendency to resist the acid wash. What three microorganisms are tested Acid-fast staining can be used to test microorganisms such as bacterial endospores, Sarcocystis, and Hydatid cysts What antibiotics are used to treat each of the three microorganisms

3 The use can treat bacterial endospores of strong oxidants like 1:10 diluted bleach; the use treats Sarcocystis of sulfadiazine, acetylspiramycis, and tinidazole antibiotics, while Hydatid cysts are treated by benzimidazole albendazole ( Bayot et al., 2019) .

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4 References Aryal S. (2022). Acid-fast stain principle, procedure, interpretation, and examples. Microbiology info.com https://microbiologyinfo.com/acid-fast-stain-principle-procedure-interpretation- and-examples/ Bayot, M. L., Mirza, T. M., & Sharma, S. (2019). Acid fast bacteria. Xiong, Y., Ba, X., Hou, A., Zhang, K., Chen, L., & Li, T. (2018). Automatic detection of mycobacterium tuberculosis using artificial intelligence. Journal of thoracic disease , 10 (3), 1936.

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