2 Lab Worksheet- 172.docx

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Student Name: Date: TA Name: Week 2 In-Lab Assignment (20 points) Bold your answers. Save as PDF. Submit via Laulima by 11:55 pm day after lab. Part I. Fungi: Division Zygomycota Figure 1 : I.s slide of Bread Mold ( Rhizopus stolonifer ) stained with Periodic Acid-Schiff(PAS) and viewed under 100x magnification with a compound microscope ; with labeled parts of the specimen. 1. Based on the structures you observed, explain what type of reproduction (asexual vs. sexual) you observed in this fungal species. Explain how this type of reproduction is used by this fungal species and refer to structures you observed in the figure above. (> 3 sentences) (1) This fungal species can use both asexual and sexual reproduction, this is shown by the presence of both the sporangia and zygosporangia; in this case the zygosporangia are more frequent in this specimen. This would mean that the Rhizopus stolonifer was not in an ideal situation to reproduce asexually and needed to undergo sexual reproduction . Part II. Fungi: Coprinua comatus Slide

Figure 2 : I.s slide of a Coprinus Mushroom (Coprinua comatus) stained and viewed under 100X magnification with a compound microscope ; with labeled parts of the specimen. Figure 2.5 : I.s slide of a Coprinus Mushroom (Coprinua comatus) stained and viewed under 400X magnification with a compound microscope ; with labeled parts of the specimen. Discussion: 1. Look up the classifications of the fungal species on this microscope slide. (0.5) Kingdom: Fungi Phylum/Division (remember this): Basidiomycota Class: Agaricomycetes Scientific name: Coprinus comatus Common Name(s): shaggy ink cap, shaggy mane Etymology of scientific name: coprinus “living on dung”. “comatus means “hairy” Part III. Lichen Figure 3 : w.m slide of an unidentified Lichen species stained and viewed under 400x magnification with a compound microscope ; with labeled parts of the specimen. Discussion: 2. Which layer is closest to the sun, the upper cortex or photosymbiont layer? Propose an explanation why. (0.5) The upper cortex is closer to the sun, this is to protect the photosynthetic organisms within the Lichen from UV damage and external resistance/s.

Part IV: Pond Water Slide Figure 4 : w.m slide of a water sample collected from a hydroponics farm located in Kaneohe, HI. Unidentified organisms circled in red and viewed under 100x magnification with a compound microscope. Discussion: 3. Looking at Figure 6 in the Lab Guide and any outside reference, explain which algal or non-algal group do you think you observed and explain reasoning. (0.5) I believe this is a species of algae belonging to the Brown Algae group(Phaeophyta). This is because the other species of freshwater algae do not match the characteristics of the shown brown algae specimens in the water sample.

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Assessment Rubric Total Possible Points Earned Points Comments (may be shared instead via Laulima in the Assignments tab) Image appearance and sized appropriately 0.5 Structures correctly labeled 7 Photos captioned correctly and with relevant information (see table below) 10 Questions 2.5 Total Points 20

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