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Student Name: Date: TA Name: 4 In-Lab Assignment (20 points) Part I. Meristematic Tissues in Plants Apical Meristems of the Root and Shoot (labeled) Figure 1.1: I.s slide of corn ( Zea mays L.) root tip viewed under 100x magnification. A.) Apical Meristem, B.) Root Cap, C.) Protoderm, D.) Ground Meristem and E.) Procambium. Figure 1.2: I.s slide of Painted Nettle( Coleus scutellariodes ) apical meristem viewed under 40x magnification. A.) Apical Meristem, B.) Leaf Primordial, C.) Axillary Bud, D.) Protoderm, E.) Ground Meristem, and F.) Procambium. 1. In complete sentences of your own words, for Figure 1, describe each labeled structure, where it is located in the figure above, and what it’s function/purpose/what it will give rise to/develop into in the plant. - The part labeled A is the apical meristem, this is responsible for forward growth of the plants roots. The root cap B, is responsible for protecting the apical meristem from obstacles and makes it easier to travel between soil. The protoderm C, is another form of protecting the plants roots and this is where lateral growth of roots and cell elongation occur. The ground meristem is responsible for producing ground tissue and vascular tissue in plants. The procambium E, is responsible for production of new cells in the plant roots. 2. In complete sentences of your own words, for Figure 2, describe each labeled structure, where it is located in the figure above, and what it’s function/what it will give rise to/develop into in the plant. - The apical meristem A, is responsible for upwards growth of the plant's stem. The lead primordia B, are a group of cells that develop into new leaves along the plant's stem. Axillary buds C, develop into leaf axils. The

protoderm D, produces cells for the epidermis which is the outermost layer of the plant. The ground meristem E, produces ground tissue like chloroplasts for photosynthesis. F, the procambium produces cells for vascular tissue in the plant. Part II. Permanent Tissues in Plants 3. Insert labeled images of dicot and monocot cross-sections, and a vascular bundle. Figure 2.1: c.s slide of unknown dicot species viewed under 40x magnification. A.) Epidermis, B.) Vascular Bundles, C.) Ground Tissue, D.) Pith, and E.) Cortex. Figure 2.2: c.s slide of unknown monocot species viewed under 40x magnification. A.) Epidermis, B.) Vascular Bundles, and C.) Ground Tissue. 4. Close-up of a vascular bundle. Figure 2.3: c.s slide of unknown dicot species viewed under 400x magnification. A.) Cortex, B.) Phloem, C.) Xylem, and D.) Pith.

Part III. Toluidine Staining in plant stem tissues 5. Insert stained and unstained images of a stem cross-section. Figure 3.1: c.s slide of Umbrella Plant( Schefflera spp. ) stem cross section viewed under 40x magnification stained with 0.08% Toluidine Blue dye. Figure 3.2: c.s slide of Umbrella Plant( Schefflera spp. ) stem cross section viewed under 40x magnification. Discussion (bold your answers) 6. Using the pre-prepared slide figures as examples, explain all the differences between tissues and tissue arrangement in monocot and dicot stems. And discuss the reason why the stem you used to make a cross-section would be considered either a monocot or dicot? - The main difference between monocots and dicots is the absence of a pith in monocots. This is what gives dicots their circular arrangement of vascular bundles whilst monocot vascular bundles are scattered throughout the ground tissue. Because of this I am fairly certain that the plant I used for a cross section is considered a dicot.

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Assessment for this assignment is found below: Assessment Rubric Total Possible Points Earned Points Comments Appearance of Images -right magnification -in focus -formatted and sized properly 1 Required Structures Identified and Labeled Correctly 9.5 Photos captioned correctly and thoroughly 3.5 Discussion questions are answered thoroughly with supporting points 6 Total Points 20

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