Virtual Microscope Tutorial (1) (1)
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Virtual Microscope Tutorial: http://www.ncbionetwork.org/iet/microscope/
Please highlight your answers or use red font color.
Navigate to the virtual microscope website (above). Begin by clicking “Guide” and reading the available information, including the section on “Terminology.” 1.
What is the magnification of the “scanning” objective lens?
4x,10x,40x,100x
2.
What is the name of the platform which holds the slides?
Stage Clips
3.
What is the function of the “condenser” on the microscope?
Objective Lens
4.
What is the “field of view”? The maximum area view when looking through a microscope with the eye piece
5.
How many micrometers (microns) are in 1 millimeter? 1000
6.
What is the typical magnification on an “oil immersion” lens?
1000 x in total power
Now close the reference guide and click on “learn”. Click on the different parts of the microscope and the other objects on the lab bench. You must click through all of the question marks to continue advancing. Hit “next” when appropriate. 7.
What difference(s) do you notice when viewing objects through the 100X lens, with vs. without oil?
With oil it is clearer and more sharp to look at, without oil it is a little harder to see as details
8.
When viewed through the 10X eyepiece of the microscope, how large will objects appear if using the scanning objective lens?
40 X
9.
An object is viewed at 400 times its actual size. The microscope used had 10X eyepieces. What was the magnification of the objective lens used?
40 x
Now click on “Explore” from the main screen. View the slides in the slide box to answer the questions.
To view the objects clearly, you will need to adjust the parameters for course focus, fine focus, and iris diaphragm aperture (light adjustment). de
10.
Use the 4X objective and 10X eyepiece. Adjust the light for the best image. Was the best image found by placing the light adjust lever left of center or right of center?
placing the light left of image 11.
Use the 4X objective and 10X eyepiece. View the letter e slide. How would you describe the orientation of the letter e on the screen? is upside down and in italics
12.
Switch to the 40X objective and 10X eyepiece. Do you need to adjust to let in more light or less light when you increased the magnification? adjust in more light when added increase on magnification 13.
Did the field of view increase or decrease when you increased the magnification?
decrease
14.
Find the slide of the onion root tip. Begin with the 4X objective but work up to the 40X objective
lens. Use the 10X eyepiece. Once cells are in proper focus and illumination, determine whether the cells are prokaryotic or eukaryotic. Explain your choice.
Prokaryotic because, the cells are small and simple in the onion root tip.
15.
What is the total magnification using the 10X eyepiece and 40X objective lens?
400
16.
How many cells fit within the field of view (approximately) at this magnification?
1
17.
Examine the slide of human compact bone using the 10X objective. Describe the shapes you are seeing.
Circles are everywhere. It reminds me of when you roll up a plato into cylinder piece and then you were to cut the cylincer piece into smaller pieces of circles. They are volume like but also circles. Explore the remaining slides at your leisure.
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Related Questions
answer the following:
instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:
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Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.
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Please see attached . Thank you
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Using the provided materials and the microscope itself, review the parts of the microscope including the name, where it is located, its function and the appropriate times to use.
Record your observations and notes as vou will require this information when writing yourlab report
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SBI 3C1
VIRTUAL LAB: THE MICROSCOPE
INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the
instructions on how to properly use a microscope. When you are complete, answer the questions below.
PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen?
TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification)
Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE
ОBJECTIVE
LENS
POWER
OCULAR
MAGNIFICATION
OBJECTIVE LENS
MAGNIFICATION
TOTAL MAGNIFICATION
MAG (X) = Ocular X Objective
LOW
LP MAG =
MEDIUM
MP MAG=
HIGH
HP MAG-
Complete the following chart by calculating the missing lens or total magnification [2]
TOTAL MAGNIFICATION
OBJECTIVE LENS MAGNIR
AR (EYEPIECE)
MAGNIFICATION
5X
80X
10X
40X
10X
100X
500X
50X
PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES
Access the Virtual Microscope at…
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Please answer question 1
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Please provide the quality assurance procedure that ensures reliable results in CLINICAL MICROSCOPY? Please answer in detail; thank you
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Can you provide short answers for these questions ?
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How do I complete this tableabout a light microscope?
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List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step.
1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage.
a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage.
4. Use the Low Power Objective.
a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…
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Complete Table 1 below by supplying the characteristics of each
objective.
Table 1. Numerical characteristics of microscope objectives.
LPO
НРО
OIO
Focal length
(mm)
Working distance
(mm)
Linear
magnification (X)
Numerical
aperture (N.A..)
Features (color
of the band,
diameter of the
opening of the
lens, etc.)
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Using a Light Microscope to Determine an Object's SIZE
PRE-LAB QUESTIONS
Fill in the diagram of the microscope with the term or description that matches, the microscope
part.
Eye Plece
Body Tube
Contains lens to increase
magnification usually 10x
Revolves to allow changing
various objectives
Arm
Objectives
Moves stage up and down
approximately to correct
distance
Hold slides in place
Stage
Permits finer focusing by
moving the stage in
smaller increments
Regulates the amount of
light going through the stage
Base
Light Source
Copyright © 2012 Laying the Foundation®, Ic., Dallas, Texas. All rights reserved. Visit us online at www.lftralning.org.
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Please include your reference below for my further research. Thank you!
1. What are the basic components of a Fluorescence Microscope and what are the functions of each?
2. Are there any parts that you can remove without compromising accuracy and utility of the equipment?
3. Can you suggest additional components to improve the equipment?
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State the function of each part of the compound light microscope.
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Please answer fast
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can you please answer these in order
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Label the parts of the microscope on the diagram below: arm, stage, base, ocular, revolving nosepiece, objective (4x, 10x, 40x), course focus, fine focus, condenser & iris diaphragm (these are marked by the same line)
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Answer these questions by watching the YouTube videos and reviewing the Powerpoints from Lab #4.
1. What is Refraction of light?
2. What is the difference between the Ocular lens and the Objective lens?
3. What is the purpose of the Revolving Nosepiece?
4. What is the difference between the Course Adjustment Knob and the Fine Adjustment Knob?
5. How do you calculate Total Magnification?
6. What is Resolution in terms of Microscopy?
7. What is the purpose of Oil Immersion?
8. What is the Diffraction Barrier and why does it exist?
9. What is the purpose of using stains and fluorescent dyes in microscopy?
10. What is the advantage of using an Electron Microscope?
11. What Objective lens should you always start with?
12. What is the purpose of the Iris Diaphragm on the Condenser?
13. How do you know your Objective lens has been adjusted properly?
14. Why should you not use Kimwipes…
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Help with
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Please answer asap and in short and content should not be palgarised please answer asap
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A. Purpose:
Figure 1
B. Materials:
Microscope
Magazine
Slides and cover slips
Paper towels
Pipette
Scissors
C. Procedure:
1. Careful carry a microscope to your lab area. Make sure to hold it with one
hand under the base and one hand on the arm as shown in Figure 1.
2. Plug the microscope in and turn it on. Take a moment to look at all the parts of the microscope. Then look
at your ocular lens. What is the magnification of the ocular lens (eye piece)?
Figure 2
3. Fill in the chart to show the total magnification for each objective lens.
Magnification
of Ocular Lens
Magnification of
Objective Lens
Objective Lens
Total
Magnification
Low Power
Medium Power
High Power
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Match the terms on the left with its description
_____ stage
_____ocular lens
____Diaphragm
_____adjustment focusing knob
_____Body tube
holds the microscope slide
lens you look through, usually 10x power
regulate the amount of light passing the specimen
used to focus on specimen
holds the eyepiece
upper part of the microscope that contains optical components.
holds the object lens
magnifies specimen
secures that slice to the stage
condenses light from the light source into a narrow beam
source of illumination
magnifies an image 100x,
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a. Why must you slowly turn the fine adjustment knob?
b. Is natural light or is artificial light used when using the microscope?
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Give a particular use/uses of the following types of a microscope:
1. Research microscope
2. Stereomicroscope
3. Student microscope
4. Projection microscope
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Can you help me to fill in the function of each, please
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Please answer the following questions (Question 1 AND 2) about compound microscopes.
After clicking the objective into position, which adjustment knob(s) can you use to safely refocus the image?
You may need to adjust the light intensity to better see the specimen. Indicate two ways in which you can change the light intensity?
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9:06
LTE
4) Answer true or false to each of the following statements.
On high power, you should use the coarse
adjustment knob.
The diaphragm determines how much light shines on
the specimen.
The low power objective has a greater magnification
than the scanning objective.
The fine focus knob visibly moves the stage up and
down.
Images viewed in the microscope will appear upside
down.
If a slide is thick, only parts of the specimen may
come into focus.
The type of microscope you are using is a scanning
microscope.
For viewing, microscope slides should be placed on
the objective.
In order to switch from low to high power, you must
rotate the revolving nosepiece.
The total magnification of a microscope is
determined by adding the ocular lens power to the
objective lens power.
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Answer the following questions:
Why are most cells so small?
Why cell size is limited?
List two instances when the coarse adjustment knob is never used
Why is immersion oil used with the 100X objective?
List two common problems associated with using the microscope and how you would go about solving it.
When should the lenses be cleaned? What is the correct way to clean them?
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Hi, there! Just subscribed to Bartleby! So excited to be here, and thanks for your help! Can you assist me in answering this? (This is not graded but, rather, an excersise conducive to acquisition).
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A
B
C
D
LEICA E
H
J
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Saved
Label the following parts of this typical light microscope.
Mechanical
stage
Light source
Ocular lens
Fine focus
Primo Star
Coarse focus
Mechanical
stage
Revolving
nosepieces
Conderser
Lamp voltage
control
Lamp voltage
control
Objective lens
Objective lens
Conderser
Ocular lens
Diaphragm
Revolving
nosepieces
Fine focus
Coarse focus
Diaphragm
Light source
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Answer the following questions and uploa
assignment.
1. What is the difference between a zoom knob and focus knob?
2. What is another name for the ocular lens?
3. Define working distance.
4. When would you use top illumination vs. bottom illumination?
5. What two parts of the microscope should you touch when carrying it?
MacBook Air
吕0
F3
F4
F5
F6
F7
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identify the parts and functions of a microscope. Provide your answers on the spaces provided below.
Fine Focus
____________________________________________________________________________________________________________________________________________________________________________________________________________________________________
Coarse Focus
____________________________________________________________________________________________________________________________________________________________________________________________________________________________________
Stage Clip
____________________________________________________________________________________________________________________________________________________________________________________________________________________________________
Rack Stop…
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Previous question:
For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope.
- The Answer I got for this question was 3/4mm.
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Write T (True) or F (False) for the following statements.
1. The objective lens is the one nearest to the observer's eye.
2. Only special lens paper should be used to clean the objectives, oculars,
and the condenser of the microscope.
3. The working distance is the distance between the specimen and the objective
lens.
_4. Magnifying a blurred image generally reveals further details.
5. When a microscope is parfocal, you should not have to use the coarse
adjustment at higher magnification.
_6. The total magnification of the object seen at high dry power is approximately
40X.
7. Immersion oil can be used to increase resolution of all the objective lenses in a
brightfield microscope.
_8. Always be sure to oil your microscope lenses before returning the instrument
to its designated space.
9. Animals will not survive without mitochondria because they will not be able
to make ATP.
10. The use of normal saline solution (NSS) in viewing cheek cells is to prevent
lysis or crenation of cells.
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- answer the following: instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:arrow_forwardShown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.arrow_forwardPlease see attached . Thank youarrow_forward
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- List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step. 1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage. a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage. 4. Use the Low Power Objective. a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…arrow_forwardComplete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)arrow_forwardUsing a Light Microscope to Determine an Object's SIZE PRE-LAB QUESTIONS Fill in the diagram of the microscope with the term or description that matches, the microscope part. Eye Plece Body Tube Contains lens to increase magnification usually 10x Revolves to allow changing various objectives Arm Objectives Moves stage up and down approximately to correct distance Hold slides in place Stage Permits finer focusing by moving the stage in smaller increments Regulates the amount of light going through the stage Base Light Source Copyright © 2012 Laying the Foundation®, Ic., Dallas, Texas. All rights reserved. Visit us online at www.lftralning.org.arrow_forward
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