Virtual Microscope Tutorial (1) (1)

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Patrick Henry Community College *

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101

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Biology

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Jun 14, 2024

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docx

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Virtual Microscope Tutorial: http://www.ncbionetwork.org/iet/microscope/ Please highlight your answers or use red font color. Navigate to the virtual microscope website (above). Begin by clicking “Guide” and reading the available information, including the section on “Terminology.” 1. What is the magnification of the “scanning” objective lens? 4x,10x,40x,100x 2. What is the name of the platform which holds the slides? Stage Clips 3. What is the function of the “condenser” on the microscope? Objective Lens 4. What is the “field of view”? The maximum area view when looking through a microscope with the eye piece 5. How many micrometers (microns) are in 1 millimeter? 1000 6. What is the typical magnification on an “oil immersion” lens? 1000 x in total power Now close the reference guide and click on “learn”. Click on the different parts of the microscope and the other objects on the lab bench. You must click through all of the question marks to continue advancing. Hit “next” when appropriate. 7. What difference(s) do you notice when viewing objects through the 100X lens, with vs. without oil? With oil it is clearer and more sharp to look at, without oil it is a little harder to see as details 8. When viewed through the 10X eyepiece of the microscope, how large will objects appear if using the scanning objective lens? 40 X 9. An object is viewed at 400 times its actual size. The microscope used had 10X eyepieces. What was the magnification of the objective lens used? 40 x Now click on “Explore” from the main screen. View the slides in the slide box to answer the questions. To view the objects clearly, you will need to adjust the parameters for course focus, fine focus, and iris diaphragm aperture (light adjustment). de
10. Use the 4X objective and 10X eyepiece. Adjust the light for the best image. Was the best image found by placing the light adjust lever left of center or right of center? placing the light left of image 11. Use the 4X objective and 10X eyepiece. View the letter e slide. How would you describe the orientation of the letter e on the screen? is upside down and in italics 12. Switch to the 40X objective and 10X eyepiece. Do you need to adjust to let in more light or less light when you increased the magnification? adjust in more light when added increase on magnification 13. Did the field of view increase or decrease when you increased the magnification? decrease 14. Find the slide of the onion root tip. Begin with the 4X objective but work up to the 40X objective lens. Use the 10X eyepiece. Once cells are in proper focus and illumination, determine whether the cells are prokaryotic or eukaryotic. Explain your choice. Prokaryotic because, the cells are small and simple in the onion root tip. 15. What is the total magnification using the 10X eyepiece and 40X objective lens? 400 16. How many cells fit within the field of view (approximately) at this magnification? 1 17. Examine the slide of human compact bone using the 10X objective. Describe the shapes you are seeing. Circles are everywhere. It reminds me of when you roll up a plato into cylinder piece and then you were to cut the cylincer piece into smaller pieces of circles. They are volume like but also circles. Explore the remaining slides at your leisure.
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