Virtual Microscope Lab: Observations and Adjustments for

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Patrick Henry Community College *

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MICROBIOLO

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Biology

Date

Jun 10, 2024

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docx

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2

Uploaded by cm2222214479

Microscope Virtual Lab Worksheet Begin by navigating your web browser to the following url: http://www1.udel.edu/biology/ketcham/microscope/scope.html Use the virtual compound microscope to practice finding the specimens on the slides and bringing them into focus. Use the checklist provided on the virtual lab to assure you are completing all the appropriate steps along the way. Answer the questions below on your worksheet. You should use a different font color, or highlight your answers to make them easier for the instructor to see. Don’t forget to save your work before uploading your file and submitting your assignment. 1. Place the letter e slide in the microscope, focus, and center the object. What do you notice about the spatial orientation of the letter e? Upside down 2. At the 10X objective, how many times its actual size does the letter e appear to your eye? (Don’t forget to account for the magnification of the objective and the ocular lenses). 100x 3. Which objectives magnify the letter e to such an extent that the entire letter is no longer visible in the field of view (you may list more than 1: 4X, 10X, 40X, 100X )? 40x, 100x 4. Observe the onion root tip slide at the 4X objective. How did you adjust the iris diaphragm to produce the clearest image ( left = closed down, middle = half open, right = open)? Closed down all the way 5. Observe the onion root tip slide at the 40X objective. How did you adjust the iris diaphragm to produce the clearest image ( left = closed down, middle = half open, right = open)? All the way open 6. In reference to question 5, did this adjustment let in more or less light than the optimum diaphragm adjustment at 4X? It let in more light. 7. Observe the onion root tip slide at the 40X objective. Approximately how many cells do you count from one end of the field of view to the other (across the diameter of what is visible)? 10 cells 8. Do these cells (refer to question 7) have nuclei, indicating that they are eukaryotic ( yes or no )? Yes
9. Observe the bacterial capsules at the 100X objective. Notice that you have a measurement device built into the right ocular lens. You can rotate this “ruler” by making a circular motion around the right eye piece with your mouse or pointer. Assuming that each tic on the ruler is equal to 1 micrometer, what is the approximate length of a typical bacterial cell in this culture in units of micrometers (measure the longest dimension of the cell)? Most are about 1micrometer 10. Observe the cheek smear slide at the 10X objective. Approximately how many individual cheek cells do you count within the red circle? 15 11. Do these human cheek cells (referring to question 10) contain nuclei, indicating that they are eukaryotic cells ( yes or no )? yes 12. Observe the cheek smear slide at the 100X objective. Notice that you have a measurement device built into the right ocular lens. You can rotate this “ruler” by making a circular motion around the right eye piece with your mouse or pointer. Assuming that each tic on the ruler is equal to 1 micrometer, what is the approximate diameter of a cheek cell in this smear, in units of micrometers (measure at the widest part of the cell)? Over 20 micrometers 13. How many times larger are you seeing these cheek cells (using the 100X objective) than their actual size? (Don’t forget to account for the magnification of the objective and the ocular lenses). 1000x
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