Virtual Microscope Lab: Observations and Adjustments for
.docx
keyboard_arrow_up
School
Patrick Henry Community College *
*We aren’t endorsed by this school
Course
MICROBIOLO
Subject
Biology
Date
Jun 10, 2024
Type
docx
Pages
2
Uploaded by cm2222214479
Microscope Virtual Lab Worksheet
Begin by navigating your web browser to the following url: http://www1.udel.edu/biology/ketcham/microscope/scope.html
Use the virtual compound microscope to practice finding the specimens on the slides and bringing them into focus. Use the checklist provided on the virtual lab to assure you are completing all the appropriate
steps along the way.
Answer the questions below on your worksheet. You should use a different font color, or highlight your answers to make them easier for the instructor to see. Don’t forget to save your work before uploading your file and submitting your assignment.
1.
Place the letter e slide in the microscope, focus, and center the object. What do you notice about the spatial orientation of the letter e?
Upside down
2.
At the 10X objective, how many times its actual size does the letter e appear to your eye? (Don’t
forget to account for the magnification of the objective and the ocular lenses).
100x
3.
Which objectives magnify the letter e to such an extent that the entire letter is no longer visible in the field of view (you may list more than 1: 4X, 10X, 40X, 100X
)?
40x, 100x
4.
Observe the onion root tip slide at the 4X objective. How did you adjust the iris diaphragm to produce the clearest image (
left
= closed down, middle
= half open, right
= open)?
Closed down all the way
5.
Observe the onion root tip slide at the 40X objective. How did you adjust the iris diaphragm to produce the clearest image (
left
= closed down, middle
= half open, right
= open)? All the way open
6.
In reference to question 5, did this adjustment let in more
or less
light than the optimum diaphragm adjustment at 4X?
It let in more light.
7.
Observe the onion root tip slide at the 40X objective. Approximately
how many cells do you count from one end of the field of view to the other (across the diameter of what is visible)?
10 cells
8.
Do these cells (refer to question 7) have nuclei, indicating that they are eukaryotic (
yes
or no
)?
Yes
9.
Observe the bacterial capsules at the 100X objective. Notice that you have a measurement device built into the right ocular lens. You can rotate this “ruler” by making a circular motion around the right eye piece with your mouse or pointer. Assuming that each tic on the ruler is equal to 1 micrometer, what is the approximate
length of a typical bacterial cell in this culture in
units of micrometers (measure the longest dimension of the cell)?
Most are about 1micrometer
10.
Observe the cheek smear slide at the 10X objective. Approximately
how many individual cheek cells do you count within the red circle?
15
11.
Do these human cheek cells (referring to question 10) contain nuclei, indicating that they are eukaryotic cells (
yes
or no
)?
yes
12.
Observe the cheek smear slide at the 100X objective. Notice that you have a measurement device built into the right ocular lens. You can rotate this “ruler” by making a circular motion around the right eye piece with your mouse or pointer. Assuming that each tic on the ruler is equal to 1 micrometer, what is the approximate
diameter of a cheek cell in this smear, in units of micrometers (measure at the widest part of the cell)?
Over 20 micrometers
13.
How many times larger are you seeing these cheek cells (using the 100X objective) than their actual size? (Don’t forget to account for the magnification of the objective and the ocular lenses).
1000x
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
- Access to all documents
- Unlimited textbook solutions
- 24/7 expert homework help
Related Questions
Please see attached . Thank you
arrow_forward
Please include your reference below for my further research. Thank you!
1. What are the basic components of a Fluorescence Microscope and what are the functions of each?
2. Are there any parts that you can remove without compromising accuracy and utility of the equipment?
3. Can you suggest additional components to improve the equipment?
arrow_forward
please help me with question
arrow_forward
Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.
arrow_forward
Please answer fast
arrow_forward
Please see attached . Thank you
arrow_forward
Can someone answer and explain the following?
What are the basic components of a Fluorescence Microscope and what are the functions of each?
Are there any parts that you can remove without compromising accuracy and utility of the equipment?
Can you suggest additional components to improve the equipment?
arrow_forward
State the function of each part of the compound light microscope.
arrow_forward
answer the following:
instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:
arrow_forward
SBI 3C1
VIRTUAL LAB: THE MICROSCOPE
INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the
instructions on how to properly use a microscope. When you are complete, answer the questions below.
PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen?
TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification)
Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE
ОBJECTIVE
LENS
POWER
OCULAR
MAGNIFICATION
OBJECTIVE LENS
MAGNIFICATION
TOTAL MAGNIFICATION
MAG (X) = Ocular X Objective
LOW
LP MAG =
MEDIUM
MP MAG=
HIGH
HP MAG-
Complete the following chart by calculating the missing lens or total magnification [2]
TOTAL MAGNIFICATION
OBJECTIVE LENS MAGNIR
AR (EYEPIECE)
MAGNIFICATION
5X
80X
10X
40X
10X
100X
500X
50X
PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES
Access the Virtual Microscope at…
arrow_forward
Write in digital format please
Describe the step-by-step process for taking micrographs on a transmission electron microscope.
arrow_forward
Complete Table 1 below by supplying the characteristics of each
objective.
Table 1. Numerical characteristics of microscope objectives.
LPO
НРО
OIO
Focal length
(mm)
Working distance
(mm)
Linear
magnification (X)
Numerical
aperture (N.A..)
Features (color
of the band,
diameter of the
opening of the
lens, etc.)
arrow_forward
What are the corresponding ring colors of the objective lenses of the microscope?
Scanner _________________________
Low Power Objective (LPO) _________________________
High Power Objective (HPO) _________________________
Oil immersion Objective (OIO) _________________________
arrow_forward
Help with
arrow_forward
Please provide the quality assurance procedure that ensures reliable results in CLINICAL MICROSCOPY? Please answer in detail; thank you
arrow_forward
Match the terms on the left with its description
_____ stage
_____ocular lens
____Diaphragm
_____adjustment focusing knob
_____Body tube
holds the microscope slide
lens you look through, usually 10x power
regulate the amount of light passing the specimen
used to focus on specimen
holds the eyepiece
upper part of the microscope that contains optical components.
holds the object lens
magnifies specimen
secures that slice to the stage
condenses light from the light source into a narrow beam
source of illumination
magnifies an image 100x,
arrow_forward
Using the microscope Answer the following questions as you work through this exercise:
1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)?
2. How is the letter “e” on the slide oriented when you see it under low or high power magnification?
3. What effect, if any, does the compound light microscope have on the orientation of the image?
arrow_forward
Please answer the following questions (Question 1 AND 2) about compound microscopes.
After clicking the objective into position, which adjustment knob(s) can you use to safely refocus the image?
You may need to adjust the light intensity to better see the specimen. Indicate two ways in which you can change the light intensity?
arrow_forward
Saved
Label the following parts of this typical light microscope.
Mechanical
stage
Light source
Ocular lens
Fine focus
Primo Star
Coarse focus
Mechanical
stage
Revolving
nosepieces
Conderser
Lamp voltage
control
Lamp voltage
control
Objective lens
Objective lens
Conderser
Ocular lens
Diaphragm
Revolving
nosepieces
Fine focus
Coarse focus
Diaphragm
Light source
arrow_forward
Explain how a microscope works by describing the functions of its optical and mechanical parts. Refer to the attached image.
arrow_forward
Which of the following is a parameter of microscopy that can affect your ability to resolve a specimen using a compound light microscope?
Question options:
Contrast
Resolving power
All of the choices
Numerical aperture
arrow_forward
Label the parts of the microscope on the diagram below: arm, stage, base, ocular, revolving nosepiece, objective (4x, 10x, 40x), course focus, fine focus, condenser & iris diaphragm (these are marked by the same line)
arrow_forward
can you please answer these in order
arrow_forward
Write process/steps on how to culture the bacillus athropaeus.
Note: please write it in the easiest way on how to culture the bacillus athropaeus because students will actually do it. Thanks!
arrow_forward
Describe the key components and principles of operation of a x-ray unit used for film/screen mammography.Use diagrams,where appropriate to illustrate your answer
arrow_forward
topic: gel electrophoresis
. What are other staining methods that can be used for PAGE?
arrow_forward
Can you help me with this question about Paramecium and Euglena?
arrow_forward
Assume your field of view under a 3X objective is 6mm across. What is the field of view in mm under a 20X objective? Show clear work with units for credit.
Field of view 20X objective? ___mm
What is the field of view of this 20X objective in micrometers?
If you observe a specimen under the 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?
arrow_forward
True or False:
Ultrathin sections are used for light microscopy but are to thin to be stable under an electron mocroscope X-ray beam.
arrow_forward
What information can be obtained from the slides prepared by wet mount and hanging drop technique for microscopic observation? Give at least three (3) information. What are the limitations of each method. (Provide 2 limitations)
arrow_forward
SEE MORE QUESTIONS
Recommended textbooks for you
Related Questions
- Please see attached . Thank youarrow_forwardPlease include your reference below for my further research. Thank you! 1. What are the basic components of a Fluorescence Microscope and what are the functions of each? 2. Are there any parts that you can remove without compromising accuracy and utility of the equipment? 3. Can you suggest additional components to improve the equipment?arrow_forwardplease help me with questionarrow_forward
- Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.arrow_forwardPlease answer fastarrow_forwardPlease see attached . Thank youarrow_forward
- Can someone answer and explain the following? What are the basic components of a Fluorescence Microscope and what are the functions of each? Are there any parts that you can remove without compromising accuracy and utility of the equipment? Can you suggest additional components to improve the equipment?arrow_forwardState the function of each part of the compound light microscope.arrow_forwardanswer the following: instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:arrow_forward
- SBI 3C1 VIRTUAL LAB: THE MICROSCOPE INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the instructions on how to properly use a microscope. When you are complete, answer the questions below. PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen? TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification) Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE ОBJECTIVE LENS POWER OCULAR MAGNIFICATION OBJECTIVE LENS MAGNIFICATION TOTAL MAGNIFICATION MAG (X) = Ocular X Objective LOW LP MAG = MEDIUM MP MAG= HIGH HP MAG- Complete the following chart by calculating the missing lens or total magnification [2] TOTAL MAGNIFICATION OBJECTIVE LENS MAGNIR AR (EYEPIECE) MAGNIFICATION 5X 80X 10X 40X 10X 100X 500X 50X PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES Access the Virtual Microscope at…arrow_forwardWrite in digital format please Describe the step-by-step process for taking micrographs on a transmission electron microscope.arrow_forwardComplete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you