Virtual Microscope Lab: Observations and Adjustments for

Using the provided materials and the microscope itself, review the parts of the microscope including the name, where it is located, its function and the appropriate times to use. Record your observations and notes as vou will require this information when writing yourlab report

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Please include your reference below for my further research. Thank you! 1. What are the basic components of a Fluorescence Microscope and what are the functions of each?   2. Are there any parts that you can remove without compromising accuracy and utility of the equipment?  3. Can you suggest additional components to improve the equipment?

Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.

Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48   Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis?   2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam?   4. What is the use of ethidium bromide and why must you wear gloves when you handle it?   5. What makes the DNA fragment move towards the positive plate?   6. What is the purpose of glycerol in the sample buffer?    7. What is the use of a DNA ladder?    8. What will happen when you increase the voltage of the set-up?   9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?

List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step. 1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage. a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage. 4. Use the Low Power Objective. a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…

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Using a Light Microscope to Determine an Object's SIZE PRE-LAB QUESTIONS Fill in the diagram of the microscope with the term or description that matches, the microscope part. Eye Plece Body Tube Contains lens to increase magnification usually 10x Revolves to allow changing various objectives Arm Objectives Moves stage up and down approximately to correct distance Hold slides in place Stage Permits finer focusing by moving the stage in smaller increments Regulates the amount of light going through the stage Base Light Source Copyright © 2012 Laying the Foundation®, Ic., Dallas, Texas. All rights reserved. Visit us online at www.lftralning.org.

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Can someone answer and explain the following? What are the basic components of a Fluorescence Microscope and what are the functions of each? Are there any parts that you can remove without compromising accuracy and utility of the equipment? Can you suggest additional components to improve the equipment?

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answer the following: instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:

SBI 3C1 VIRTUAL LAB: THE MICROSCOPE INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the instructions on how to properly use a microscope. When you are complete, answer the questions below. PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen? TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification) Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE ОBJECTIVE LENS POWER OCULAR MAGNIFICATION OBJECTIVE LENS MAGNIFICATION TOTAL MAGNIFICATION MAG (X) = Ocular X Objective LOW LP MAG = MEDIUM MP MAG= HIGH HP MAG- Complete the following chart by calculating the missing lens or total magnification [2] TOTAL MAGNIFICATION OBJECTIVE LENS MAGNIR AR (EYEPIECE) MAGNIFICATION 5X 80X 10X 40X 10X 100X 500X 50X PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES Access the Virtual Microscope at…

Write in digital format please Describe the step-by-step process for taking micrographs on a transmission electron microscope.

Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)

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Please provide the quality assurance procedure that ensures reliable results in CLINICAL MICROSCOPY? Please answer in detail; thank you

The following questions talk about increasing magnification in microscopes. Please answer questions 1 and 2 After a specimen is in focus, where in the field of view should position the specimen before increasing the magnification (be precise)? (QUESTION 2 HAS TWO PARTS TO IT): To increase the magnification, which objective should you click into position next?  After refocusing the specimen with the new objective, if you want to increase the magnification even more, which objective should you now click into position?

A microscope is turned on, under low power, with a slide positioned on the stage ready to focus.  The specimen is visible but not in focus at all.  Use the space below to write the correct procedural steps used to find and focus on the specimen under medium power using one of the microscopes in our room.

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The compound (bright-field) microscope is parfocal. It means that:   Question 4 options:   objective lenses are built into revolving nosepiece   its lenses effectively decrease focal lenght   can achieve maximum resolution lower than 0.2 μm   image should remain in focus when objective lens is changed

Please answer the following questions (Question 1 AND 2) about compound microscopes. After clicking the objective into position, which adjustment knob(s) can you use to safely refocus the image? You may need to adjust the light intensity to better see the specimen. Indicate two ways in which you can change the light intensity?