Pearson eText Microbiology: An Introduction -- Instant Access (Pearson+)
13th Edition
ISBN: 9780135789377
Author: Gerard Tortora, Berdell Funke
Publisher: PEARSON+
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Textbook Question
Chapter 9, Problem 8MCQ
A population of cells carrying a desired plasmid.
- a. antisense
- b. clone
- c. library
- d. Southern blot
- e. vector
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Plasmid vectors have the following characteristics, except _________ .
a. MCS
b. expression
c. transfection
d. single stranded
e. cloning
Match items in Column A with most related ones in B. Write the letters on the space provided.
In the 1953 study by Hershey and Chase
A. 32P was found in supernatants after T2 infection
B. 35S was found in bacterial pellets after T2 infection
C. E. coli genomes were inherited after T2 infection
D. RNA was excluded as THE heritable material
E. T2 coats were removed with a blender after initial phage binding
Chapter 9 Solutions
Pearson eText Microbiology: An Introduction -- Instant Access (Pearson+)
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Plasmids are important for recombining DNA. B.) What are the requirments of an expression vector.arrow_forwardWhich nucleic-acid based technique will detect norovirus? A. Fluorescent In Situ Hybridization (FISH) probes B. PCR with primers specific for the genome of this virus C. Terminal Restriction Fragment Length Polymorphism (T-RFLP) D. reverse transcription PCR with specific primers A patient's blood sample tests positive for the antigen to Vibrio cholerae. Which of the following interpretations is correct? A. This person has cholera. B. This person was exposed to any species of Vibrio. C. This person was exposed to V. cholerae recently. D. This person had cholera at some point in their lifetime.arrow_forwardWhich of the following may be used as a vector in a gene-cloningexperiment?a. mRNA c. Virusb. Plasmid d. Both b and carrow_forward
- Which of the following is a concern of in-vivo gene therapy? A. Lytic vector expression B. Integration into target region C. Immune response to viral vector D. Production of chimeraarrow_forwardA Ti plasmid carrying a foreign gene is inserted into which bacterium that infects a plant cell? a. Verticillium b. Arabidopsis thaliana c. Proteobacteria d. Agrobacterium tumefaciens e. Phytomyxeaarrow_forwardMatch items in Column A with most related ones in B. Write the letters on the space provided.arrow_forward
- Choose the one answer that fits best. The pGLO plasmid you used contains an araC gene, a GFP gene and a bla gene. If you performed the exact same experiment with a plasmid that contained a GFP gene and a bla gene, but was missing the araC gene, what would you expect to see on your four plates? a. The positive control would not have any bacterial lawn b. The negative control would have as many colonies as the experimental plate c. The LB/Amp/Ara plate would have some colonies but they would not be fluorescent under UV light d. The experimental plate would not have any colonies e. The LB/Amp plate containing E. coli (+)pGLO would be fluorescentarrow_forwardDrug-resistance plasmids could be transferred in A. baumannii bywhich of the following mechanisms:a. conjugation pilus d. a and bb. bacteriophages e. a, b, and cc. capture by cell wallarrow_forwardWhich of the following statements is false? a. Plasmids can be exchanged between different species of bacteria. b. The blue circle represents a small chromosome. c. The blue circle is a plasmid that contains genes that confer resistance to specific antiobiotics. d. The blue circle represents a double-stranded molecule of DNA.arrow_forward
- Transformation of mammalian cells with viruses and obtaining mammalian cells harboring the virus is a method used to maintain the virus of interest. During this process what are the plaques observed? A. Clear areas in a layer of cultured cells degenerated and lysed due to virus infection. B. Stained areas in a cell culture indicating cells infected by a virus. C. Virus colonies on agar. D. Bacterial colonies on agararrow_forwardIn the phage titer experiment, why did you plate multiple dilutions? a. so that you should have a countable plate b. to have duplicates to count c. As a control d. to be certain the E. coli was not previously infectedarrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forward
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