Lehninger Principles of Biochemistry 7E & SaplingPlus for Lehninger Principles of Biochemistry 7E (Six-Month Access)
Lehninger Principles of Biochemistry 7E & SaplingPlus for Lehninger Principles of Biochemistry 7E (Six-Month Access)
7th Edition
ISBN: 9781319125882
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 9, Problem 7P
Summary Introduction

Interpretation:

The reason for a plant engineered to express green fluorescent protein when grown in ordinary garden soil and watered normally, will glow in the dark, whereas a plant engineered to express fire fly luciferase will not.

Introduction:

Green fluorescent protein GFP was isolated from the jelly fish in1992, this protein is used as a tag or a marker for the detection of a particular protein. The GFP requires UV light for activation and this protein is important because it does not require any interactive molecule for its function. Luciferase gene was isolated from firefly and it does not need any source of light for its expression but requires a substrate for activation.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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