The ratio of adenine to thymine in a sample of a short dodacamer of DNA that contains 12 base pairs.

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Answer 1

Question

Chapter 9, Problem 79E

(a)

Interpretation Introduction

To determine: The ratio of adenine to thymine in a sample of a short dodacamer of DNA that contains 12 base pairs.

(b)

Interpretation Introduction

To determine: The ratio of cytosine to guanine in a sample of short dodacamer of DNA that contains 12 base pairs.

(c)

Interpretation Introduction

To determine: The number of sodium ions per dodecemer, assuming that the counter ions present in the solution are sodium ions and the 5' end phosphates each bear a −1 charge.

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1. Calculate the accurate monoisotopic mass (using all 1H, 12C, 14N, 160 and 35CI) for your product using the table in your lab manual. Don't include the Cl, since you should only have [M+H]*. Compare this to the value you see on the LC-MS printout. How much different are they? 2. There are four isotopic peaks for the [M+H]* ion at m/z 240, 241, 242 and 243. For one point of extra credit, explain what each of these is and why they are present. 3. There is a fragment ion at m/z 184. For one point of extra credit, identify this fragment and confirm by calculating the accurate monoisotopic mass. 4. The UV spectrum is also at the bottom of your printout. For one point of extra credit, look up the UV spectrum of bupropion on Google Images and compare to your spectrum. Do they match? Cite your source. 5. For most of you, there will be a second chromatographic peak whose m/z is 74 (to a round number). For one point of extra credit, see if you can identify this molecule as well and confirm by…

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Answer 2

Question

Chapter 9, Problem 79E

(a)

Interpretation Introduction

To determine: The ratio of adenine to thymine in a sample of a short dodacamer of DNA that contains 12 base pairs.

(b)

Interpretation Introduction

To determine: The ratio of cytosine to guanine in a sample of short dodacamer of DNA that contains 12 base pairs.

(c)

Interpretation Introduction

To determine: The number of sodium ions per dodecemer, assuming that the counter ions present in the solution are sodium ions and the 5' end phosphates each bear a −1 charge.

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Answer 3

Question

Chapter 9, Problem 79E

(a)

Interpretation Introduction

To determine: The ratio of adenine to thymine in a sample of a short dodacamer of DNA that contains 12 base pairs.

(b)

Interpretation Introduction

To determine: The ratio of cytosine to guanine in a sample of short dodacamer of DNA that contains 12 base pairs.

(c)

Interpretation Introduction

To determine: The number of sodium ions per dodecemer, assuming that the counter ions present in the solution are sodium ions and the 5' end phosphates each bear a −1 charge.

Expert Solution & Answer

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Answer 4

Question

Chapter 9, Problem 79E

(a)

Interpretation Introduction

To determine: The ratio of adenine to thymine in a sample of a short dodacamer of DNA that contains 12 base pairs.

(b)

Interpretation Introduction

To determine: The ratio of cytosine to guanine in a sample of short dodacamer of DNA that contains 12 base pairs.

(c)

Interpretation Introduction

To determine: The number of sodium ions per dodecemer, assuming that the counter ions present in the solution are sodium ions and the 5' end phosphates each bear a −1 charge.

Expert Solution & Answer

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Answer 5

Chapter 9, Problem 79E

(a)

Interpretation Introduction

To determine: The ratio of adenine to thymine in a sample of a short dodacamer of DNA that contains 12 base pairs.

(b)

Interpretation Introduction

To determine: The ratio of cytosine to guanine in a sample of short dodacamer of DNA that contains 12 base pairs.

(c)

Interpretation Introduction

To determine: The number of sodium ions per dodecemer, assuming that the counter ions present in the solution are sodium ions and the 5' end phosphates each bear a −1 charge.

Answer 6

Chapter 9, Problem 79E

(a)

Interpretation Introduction

To determine: The ratio of adenine to thymine in a sample of a short dodacamer of DNA that contains 12 base pairs.

Answer 7

Chapter 9, Problem 79E

(a)

Interpretation Introduction

To determine: The ratio of adenine to thymine in a sample of a short dodacamer of DNA that contains 12 base pairs.

Answer 8

(b)

Interpretation Introduction

To determine: The ratio of cytosine to guanine in a sample of short dodacamer of DNA that contains 12 base pairs.

Answer 9

(b)

Interpretation Introduction

To determine: The ratio of cytosine to guanine in a sample of short dodacamer of DNA that contains 12 base pairs.

Answer 10

(c)

Interpretation Introduction

To determine: The number of sodium ions per dodecemer, assuming that the counter ions present in the solution are sodium ions and the 5' end phosphates each bear a −1 charge.

Answer 11

(c)

Interpretation Introduction

To determine: The number of sodium ions per dodecemer, assuming that the counter ions present in the solution are sodium ions and the 5' end phosphates each bear a −1 charge.

Answer 12

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Answer 13

Expert Solution & Answer

Answer 14

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Answer 15

Ch. 9.2 - Prob. 9.1.1PE Ch. 9.2 - Prob. 9.1.2PE Ch. 9.2 - Prob. 9.2.1PE Ch. 9.2 - Prob. 9.2.2PE Ch. 9.2 - Prob. 9.3.1PE Ch. 9.2 - Prob. 9.3.2PE Ch. 9.3 - Prob. 9.4.1PE Ch. 9.3 - Prob. 9.4.2PE Ch. 9.5 - Prob. 9.5.1PE Ch. 9.5 - Prob. 9.5.2PE

The ratio of adenine to thymine in a sample of a short dodacamer of DNA that contains 12 base pairs.

Concept explainers

To determine: The ratio of adenine to thymine in a sample of a short dodacamer of DNA that contains 12 base pairs.

To determine: The ratio of cytosine to guanine in a sample of short dodacamer of DNA that contains 12 base pairs.

To determine: The number of sodium ions per dodecemer, assuming that the counter ions present in the solution are sodium ions and the 5' end phosphates each bear a −1 charge.

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Chapter 9 Solutions