Interpretation:
The result of the use of a large quantity of petroleum ether alone as an eluent in either experiment 1 or 2 should be determined.
Concept introduction:
A separation technique used for the segregation of components of chemical mixture based on the difference between the adsorptionof different components in stationary and mobile phase is called column chromatography.
It is used for purification or segregation of compounds. It consists of two phases namely:
- Stationary phase (solid)
- Mobile phase (generally liquid)
Silica gel is commonly used as the stationary phase.
Elution is the process of extraction of one component from another with the help of the solvent. Eluent is the carrier part of mobile phase. Eluate is the material that comes up through the chromatogram.
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EBK MACROSCALE AND MICROSCALE ORGANIC E
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