Biochemistry
Biochemistry
6th Edition
ISBN: 9781337359573
Author: Reginald H. Garrett; Charles M. Grisham
Publisher: Cengage Learning US
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Chapter 9, Problem 3P

Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.

  1. Understanding the Densities of Membrane Components Sucrose gradients for separation of membrane proteins must be able to separate proteins and protein—lipid complexes with a wide range of densities, typically 1.00 to 1.35 g/mL.

a. Consult reference books (such as the CRC Handbook of Biochemistry ) and plot the density of sucrose

solutions versus percent sucrose by weight (g sucrose per 100 g solution), and versus percent by volume (g sucrose per 100 mL solution). Why is one pint linear and the other plot curved?

b. What would be a suitable range of sucrose concentrations for Separation of three membrane-derived protein—lipid examples with densities of 1.03, 1.07, and 1.08 g/mL?

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