Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 8.4, Problem 1CC
Retrieve, Infer, Apply
1. Describe how an autoclave works. What conditions are required for sterilization by moist heat? What three things must one do when operating an autoclave to help ensure success?
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Chapter 8 Solutions
Prescott's Microbiology
Ch. 8.1 - MICRO INQUIRY Which types of agents can be used...Ch. 8.1 - Retrieve, Infer, Apply 1. Define the following...Ch. 8.1 - Retrieve, Infer, Apply 2. What is the difference...Ch. 8.2 - MICRO INQUIRY Examine graph (a). How long would it...Ch. 8.3 - MICRO INQUIRY How might one verify that filtration...Ch. 8.3 - Retrieve, Infer, Apply 1. What are depth filters...Ch. 8.3 - Retrieve, Infer, Apply 2. Describe the operation...Ch. 8.4 - Retrieve, Infer, Apply 1. Describe how an...Ch. 8.4 - Retrieve, Infer, Apply 2. In the past, spoiled...Ch. 8.4 - Prob. 3CC
Ch. 8.4 - Retrieve, Infer, Apply 2. What is the correlation...Ch. 8.5 - MICRO INQUIRY Why is it important that all of...Ch. 8.5 - MICRO INQUIRY Why are cross-linking agents such as...Ch. 8.5 - Retrieve, Infer, Apply 1. Why are most...Ch. 8.5 - Retrieve, Infer, Apply Construct a table that...Ch. 8.5 - Retrieve, Infer, Apply 3. Which disinfectants or...Ch. 8.5 - Retrieve, Infer, Apply 4. How do phenolic agents...Ch. 8.5 - Retrieve, Infer, Apply 5. Which physical or...Ch. 8.6 - Retrieve, Infer, Apply 1. Briefly explain how the...Ch. 8.6 - Retrieve, Infer, Apply How does being in a biofilm...Ch. 8.6 - Retrieve, Infer, Apply Suppose hospital custodians...Ch. 8.6 - Retrieve, Infer, Apply Briefly describe the phenol...Ch. 8.6 - Retrieve, Infer, Apply Why might it be necessary...Ch. 8.7 - Retrieve, Infer, Apply How would you explain to a...Ch. 8.7 - Retrieve, Infer, Apply Propose the use of specific...Ch. 8 - Prob. 1RCCh. 8 - Throughout history, spices have been used as...Ch. 8 - Design an experiment to determine whether an...Ch. 8 - Suppose you tested the effectiveness of...Ch. 8 - Prob. 4AL
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- Biochemical tests such as the fermentation test can be used when unknown bacterial cells are identified. true or false?arrow_forwardQ1: Why are E.coli cells subjected to heat shock induction when the optical density of the bacterial culture has reached 0.6-0.8? Q2: Why are these wash steps critical for the preparation of protein samples used in 2D gel electrophoresis?arrow_forwardMicrobial sensivity lab: Why are Escherichia coli, Pseudomonas aeruginosa, and StaphyLococcus aureus used as standards in the Kirby Bauer method?arrow_forward
- Explain about different types of bioreactors and how they are used in cell suspension culture. What are the advantage and limitations of each bioreactors?arrow_forwarda.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?arrow_forwardCitrate test What will happen to the organism if it can't use the carbon source provided?arrow_forward
- Question: Give the importance of biochemical test for the identification of the members of Enterobacteriaceae.arrow_forward281 Customized Subject Test 50. During an experiment, equal aliquots of Escherichia coli and Staphylococcus aureus are separately sonicated at the same level to mechanically disrupt bacterial cells. E. coli cells are disrupted, but there is minimal disruption of S. aureus cells. Which of the following bacterial structures best explains this difference? A) Cytoplasmic membrane B) Outer membrane C) Peptidoglycan layer OD) Phospholipid bilayer OE) Polysaccharide capsulearrow_forwardCitrate test What is the only souce of carbon in this medium?arrow_forward
- Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurredarrow_forwardGive 2 fluorogenic methods that can be used in detecting coliforms and E.coli.arrow_forwardВackground In order to determine whether a newly synthesized chemical might be a useful food preservative, the chemical was tested for its ability to inhibit bacterial growth. Control. 500 ml of cottage cheese was inoculated with 2 ml of a 24-hr culture of Pseudomonas aeruginosa and incubated at 25°C. Five hours after inoculation, a standard plate count showed there were 200 bacterial cells/ml in the cottage cheese. After 11 hours, 1600 cells/ml. After 21 hours, 50,000 cells/ml. After 27 hours, 400,000 cells/ml. Experiment. 500 ml of cottage cheese containing the preservative was inoculated with 2 ml of a 24-hr culture of P. aeruginosa. After 6 hours of incubation at 25°C, a standard plate count was performed. There were 700 bacterial cells/ml in the cottage cheese. After 14 hours, 11,000 cells/ml. After 20 hours, 90,000 cells/ml. After 28 hours, 1, 400, 000 cells/ml. Use the data and the semi-log graph paper to plot growth curves for the control and the experiment. Remember to label…arrow_forward
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