Materials Science and Engineering
Materials Science and Engineering
9th Edition
ISBN: 9781118324578
Author: Jr. William D. Callister
Publisher: WILEY
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Chapter 7.13, Problem 40QP
To determine

The time required for a metal alloy specimen to achieve a grain diameter of 8.7×102mm .

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You are asked to separate two proteins via size exclusion chromatography. Your column is 5 cm in diameter and 25 cm in length. You add 50 g (dry weight) of Sephacryl gel (density = 1.25 g/cm³) to the column. The volume outside of the gel and pores is 77 cm³. The column operates at a flow rate of 1 mL/min to not compress the resin. The average retention times of proteins A and B are 244.5 and 273 min, respectively. Under the given conditions, you calculate that there are 1600 theoretical plates in the column. (This problem continues onto the next page). a) What is the volume inside of the pores? b) What are the elution limits of this system (i.e., what re the minimum and maximum volumes that particles will elute in)? c) What are the gel partition coefficients of proteins A and B for this system? d) What are the peak widths for both proteins? e) Assuming the peak widths are 22 mL and 28 mL for proteins A and B, respectively (these values are not necessarily correct), what is the…
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1) Indicate whether each statement below is True or False: a) In extractions, small proteins usually end up in the heavy phase. b) In extractions, large proteins usually end up in the heavy phase. c) Tie line length on a phase diagram has no effect on protein partitioning. d) For extraction, we assume that each stage reaches equilibrium. e) Isotherms that are concave down lead to self-sharpening solute fronts in adsorption. f) When scaling up an adsorption unit, the LUB scales proportionally with adsorber length. g) For chromatography, separation efficiency increases when HETP increases. h) In size exclusion chromatography, smaller molecules elute from the column first. i) In hydrophobic interaction chromatography, very hydrophobic proteins elute in water. j) Molecules at very high concentrations elute from the column as a perfect Gaussian peak. k) In chromatography scale-up, resolution will increase as column diameter increases. 1) A good adsorbent typically has no porosity. m)…
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