Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 6, Problem 3CT
Summary Introduction
To write:
Acidic congo red does not have the suspended particles unlike the nigrosin butprovides the presence of a negative stain. Give the basis for this stain.
Introduction:
Heat fixing:
After the drying of smear at the room temperature on the slide. This slide is gripped with the help of tongs or a clothespin. Then it is passed across the flame of a Bunsen burner and numerous times to heat-kill. It attaches the organism to the slide. Heat fixation usually conserves generally the morphology but not an internal structure.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect
the staining of bacteria?
Can dyes other than methylene blue be used for direct staining? Briefly explain.
What is the principle of Giemsa staining and components of Giemsa stain?
What is an example of a negative stain?
Chapter 6 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.arrow_forwardIs it possible to perform double or triple staining using fluorescent dyes? Explain.arrow_forwardCrystal violet is an example of what type of stain?arrow_forward
- For What purpose is Giemsa stain used for?arrow_forwardWhy is it called as differential stainarrow_forwardExplain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayarrow_forward
- For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safraninarrow_forwardDescribe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?arrow_forwardDifferent stains have different affinities for different organisms or different parts of organisms. Elaborate on this statement using the Hematoxylin and Eosin staining as an example.arrow_forward
- Why is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.arrow_forwardThe bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?arrow_forwardWhat are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you