EBK MICROBIOLOGY
12th Edition
ISBN: 8220100659720
Author: CASE
Publisher: PEARSON
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Chapter 6, Problem 3A
Most laboratory media contain a fermentable carbohydrate and peptone because the majority of bacteria require carbon, nitrogen, and energy sources in these forms. How are these three needs met by glucose-minimal salts medium? (Hint: See Table 6.2.)
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The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5g * L ^ - 1 extract, 3g * L ^ - 1 yeast extract and 5g * L ^ - 1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. marks)
a) How would you prepare a 500 ml nutrient broth?
b) How would you prepare 2% (w / v) nutrient agar for 1L?
c) How would you prepare a 100 ml nutrient broth supplemented with 100mu * g / m * l ampicillin antibiotic? The stock concentration for ampicillin solution is 100mg / m * l .
You inoculate two test tubes with the same amount of identical growth medium and with the same number of
identical yeast cells and grow these cells under identical conditions except for the presence or absence of
oxygen.
b) The cells of both cultures convert glucose to pyruvate via glycolysis, and then further metabolize pyruvate.
Are the cells of both cultures able to obtain the same amount of ATP from glycolysis? Explain.
Under anaerobic conditions, the carbon from pyruvate will ultimately be found in which molecule?
Under aerobic conditions, the carbon from pyruvate will ultimately be found in which molecule?
Menaquinones is produced by Flavobacterium meningosepticum via submerged fermentation with 60% working volume in 30-L fermentation. To obtain the crude menaquinones, the fermentation medium is centrifuged at 12,000 rpm for 15 minutes. The vacuum-freeze dried particles are crushed and collected. After that the samples are treated with methanol for 20 minutes. To remove polar lipid compounds from bacterial cells, HZ816 macroporous adsorption resin column is used with methanol-dichloromethane (l:1) as mobile phase and flow rate of 0.75 mL/min. Rotary evaporation at 500 C is carried out to concentrate the eluent. After that, the concentrated sample is purified with Bio-BeadsTM S-X3 to remove components without UV absorption. To obtain the homolog purified menaquinones, RP-C18 column is used. Recrystallization is carried out to obtain pure crystalized menaquinones.
Answer the following:
Indicate how to obtain the sample in the methanol solvent.
Three different chromatography are used in…
Chapter 6 Solutions
EBK MICROBIOLOGY
Ch. 6 - Prob. 1RCh. 6 - Prob. 2RCh. 6 - Prob. 3RCh. 6 - Seven methods of measuring microbial growth were...Ch. 6 - By deep-freezing, bacteria can be stored without...Ch. 6 - A pastry chef accidentally inoculated a cream pie...Ch. 6 - Nitrogen and phosphorus added to beaches following...Ch. 6 - Differentiate complex and chemically defined...Ch. 6 - DRAW IT Draw the following growth curves for E....Ch. 6 - NAME IT A prokaryotic cell hitched a ride to Earth...
Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Assume you inoculated 100 facultatively anaerobic...Ch. 6 - The term trace elements refers to a. the elements...Ch. 6 - Which one of the following temperatures would most...Ch. 6 - Which of the following is not a characteristic of...Ch. 6 - Which of the following types of media would not be...Ch. 6 - An organism that has peroxidase and superoxide...Ch. 6 - E. coli was incubated with aeration in a nutrient...Ch. 6 - Clostridium and Streptococcus are both...Ch. 6 - Most laboratory media contain a fermentable...Ch. 6 - Flask A contains yeast cells in glucose-minimal...Ch. 6 - Clinical Applications and Evaluation 1. Assume...Ch. 6 - Heat lamps are commonly used to maintain foods at...Ch. 6 - The number of bacteria in saliva samples was...
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