Chapter 6, Problem 10P

(a)

Summary Introduction

To calculate: The K_cat of enzyme.

Introduction:

Enzyme kinetics is defined as the study of enzyme catalyzed chemical reactions. It indicates a rate of reaction as a function of substrate concentration with $V_{\max }$ and $K_m$ of enzyme.

(b)

Summary Introduction

To calculate: The values of V_max and K_m that will be observed in the presence of sufficient amount of an uncompetitive inhibitor to generate an ${\text{α}}^{\prime }$ of 2.0.

Introduction:

Inhibitor is a chemical molecule that may inhibit the activity of enzyme by binding at its active site. It prevents the binding of substrate with enzyme. There are two types of inhibitors such as reversible and irreversible inhibitors.