Campbell Biology
Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Chapter 56, Problem 56.4CR
Summary Introduction

To analyze: The reason to choose a healthier way to feed at a lower or a higher trophic level due to biological magnification caused by the accumulation of toxins.

Introduction: The study of the life cycle includes the effect of the accumulation of the toxic effects of pesticides at each trophic level of the biological chain. The first organisms include the bacteria and other microorganisms, which metabolize the harmful pesticides and chemicals. The levels of the accumulation are analyzed by calculating the concentration in parts per million (ppm).

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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