Anatomy & Physiology: The Unity of Form and Function
8th Edition
ISBN: 9781259277726
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 5.1, Problem 3BYGO
Summary Introduction
Introduction:
A germ cell is considered as the embryonic precursor where the development of human begins. Different germ layers are formed in a fertilized embryo and contribute to the development of tissues and organs through the process of gastrulation.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 5 Solutions
Anatomy & Physiology: The Unity of Form and Function
Ch. 5.1 - Classify each of the following into one of the...Ch. 5.1 - What are tissues composed of in addition to cells?Ch. 5.1 - Prob. 3BYGOCh. 5.1 - Prob. 4BYGOCh. 5.1 - Two names for the branch of biology concerned with...Ch. 5.1 - Prob. 2AYLOCh. 5.1 - The roles of cells, matrix, fibers, and ground...Ch. 5.1 - Primary germ layers of the embryo and their...Ch. 5.1 - Prob. 5AYLOCh. 5.2 - Distinguish between simple and stratified...
Ch. 5.2 - Prob. 6BYGOCh. 5.2 - Prob. 7BYGOCh. 5.2 - Prob. 8BYGOCh. 5.2 - Prob. 1AYLOCh. 5.2 - Prob. 2AYLOCh. 5.2 - 5.2 Epithelial Tissue 3. Defining characteristics...Ch. 5.2 - Prob. 4AYLOCh. 5.2 - Prob. 5AYLOCh. 5.2 - Prob. 6AYLOCh. 5.2 - Prob. 7AYLOCh. 5.2 - Prob. 8AYLOCh. 5.2 - Prob. 9AYLOCh. 5.3 - What features do most or all connective tissues...Ch. 5.3 - Prob. 10BYGOCh. 5.3 - Prob. 11BYGOCh. 5.3 - Prob. 12BYGOCh. 5.3 - Discuss the difference between dense regular and...Ch. 5.3 - Prob. 14BYGOCh. 5.3 - Prob. 15BYGOCh. 5.3 - Prob. 1AYLOCh. 5.3 - Prob. 2AYLOCh. 5.3 - Prob. 3AYLOCh. 5.3 - Prob. 4AYLOCh. 5.3 - Prob. 5AYLOCh. 5.3 - 5.3 Connective Tissue 6. The appearance,...Ch. 5.3 - Prob. 7AYLOCh. 5.3 - Prob. 8AYLOCh. 5.3 - Prob. 9AYLOCh. 5.3 - Prob. 10AYLOCh. 5.3 - 5.3 Connective Tissue 11. Why blood is classified...Ch. 5.4 - Prob. 16BYGOCh. 5.4 - Prob. 17BYGOCh. 5.4 - Prob. 18BYGOCh. 5.4 - Prob. 1AYLOCh. 5.4 - Prob. 2AYLOCh. 5.4 - Prob. 3AYLOCh. 5.4 - 5.4 Nervous and Muscular TissuesExcitable Tissues...Ch. 5.4 - Prob. 5AYLOCh. 5.5 - Prob. 19BYGOCh. 5.5 - Prob. 20BYGOCh. 5.5 - Prob. 21BYGOCh. 5.5 - Prob. 22BYGOCh. 5.5 - Prob. 23BYGOCh. 5.5 - Prob. 1AYLOCh. 5.5 - Prob. 2AYLOCh. 5.5 - Prob. 3AYLOCh. 5.5 - Prob. 4AYLOCh. 5.5 - Prob. 5AYLOCh. 5.5 - Prob. 6AYLOCh. 5.5 - Prob. 7AYLOCh. 5.5 - 5.5 Cell Junctions, Glands, and Membranes 8....Ch. 5.5 - 5.5 Cell Junctions, Glands, and Membranes 9....Ch. 5.5 - Prob. 10AYLOCh. 5.6 - Distinguish between differentiation and...Ch. 5.6 - Prob. 25BYGOCh. 5.6 - Prob. 26BYGOCh. 5.6 - Distinguish between regeneration and fibrosis....Ch. 5.6 - Prob. 1AYLOCh. 5.6 - Prob. 2AYLOCh. 5.6 - 5.6 Tissue Growth, Development, Repair, and...Ch. 5.6 - Prob. 4AYLOCh. 5.6 - Prob. 5AYLOCh. 5.6 - Prob. 6AYLOCh. 5.6 - Prob. 7AYLOCh. 5.6 - 5.6 Tissue Growth, Development, Repair, and...Ch. 5 - Transitional epithelium is found is a. the urinary...Ch. 5 - Prob. 2TYRCh. 5 - Which of these is a primary germ layer? a....Ch. 5 - A seminiferous tubule of the testis is lined with...Ch. 5 - Prob. 5TYRCh. 5 - A fixative serves to a. stop tissue decay. b....Ch. 5 - Prob. 7TYRCh. 5 - Prob. 8TYRCh. 5 - Prob. 9TYRCh. 5 - Prob. 10TYRCh. 5 - Prob. 11TYRCh. 5 - Prob. 12TYRCh. 5 - Prob. 13TYRCh. 5 - Prob. 14TYRCh. 5 - Tendons and ligaments are made mainly of the...Ch. 5 - Prob. 16TYRCh. 5 - Prob. 17TYRCh. 5 - Fibers and ground substance make up the ______ of...Ch. 5 - A/An _______ adult stem cell can differentiate...Ch. 5 - Prob. 20TYRCh. 5 - Prob. 1BYMVCh. 5 - Prob. 2BYMVCh. 5 - State a meaning of each word element, and give a...Ch. 5 - Prob. 4BYMVCh. 5 - Prob. 5BYMVCh. 5 - State a meaning of each word element, and give a...Ch. 5 - Prob. 7BYMVCh. 5 - Prob. 8BYMVCh. 5 - Prob. 9BYMVCh. 5 - Prob. 10BYMVCh. 5 - The esophagus is protected from abrasion by a...Ch. 5 - Prob. 2WWTSCh. 5 - Prob. 3WWTSCh. 5 - Prob. 4WWTSCh. 5 - Prob. 5WWTSCh. 5 - Prob. 6WWTSCh. 5 - Prob. 7WWTSCh. 5 - Prob. 8WWTSCh. 5 - Prob. 9WWTSCh. 5 - Prob. 10WWTSCh. 5 - A woman in labor is often told to push. In doing...Ch. 5 - A major tenet of the cell theory is that all...Ch. 5 - Prob. 3TYCCh. 5 - The epithelium of the respiratory tract is mostly...Ch. 5 - Which do you think would heal faster, cartilage or...
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