BROOKER BIO 3-HOLE PUNCH W/CONNECT BUND
5th Edition
ISBN: 9781264065615
Author: BROOKER
Publisher: MCGRAW-HILL LEARNING SOLN.(CC)
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Chapter 50.3, Problem 1EQ
Summary Introduction
To describe: The procedure implemented by Banting and Best to obtain glucose-lowering factor produced by the pancreas.
Introduction: Frederick Banting and his student Charles Best proposed a procedure to obtain glucose lowering factor produced by the pancreas. Prior to their research work, the researchers assumed that glucose lowering factor was the enzymes released from the exocrine portion of the pancreas.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 50 Solutions
BROOKER BIO 3-HOLE PUNCH W/CONNECT BUND
Ch. 50.2 - Prob. 1CSCh. 50.3 - Prob. 1CSCh. 50.3 - Prob. 1EQCh. 50.3 - Prob. 2EQCh. 50.3 - Prob. 3EQCh. 50.4 - Prob. 1CCCh. 50.4 - Prob. 1CSCh. 50.4 - Prob. 2CCCh. 50.5 - Prob. 1CCCh. 50.5 - Prob. 2CC
Ch. 50 - Which is the defining feature of hormones? a. They...Ch. 50 - Steroid hormones are synthesized from ______blank...Ch. 50 - Prob. 3TYCh. 50 - Prob. 4TYCh. 50 - Prob. 5TYCh. 50 - Prob. 6TYCh. 50 - Which of the following pairs of hormones are...Ch. 50 - In invertebrates, molting of larvae is stimulated...Ch. 50 - Which of the following is true of the adrenal...Ch. 50 - Prob. 10TYCh. 50 - Prob. 1CQCh. 50 - Distinguish between type 1 and type 2 diabetes...Ch. 50 - Core Concept: Systems Organ systems often exert...Ch. 50 - Discuss the roles of hormones in growth and...Ch. 50 - Prob. 2COQ
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