BIOCHEMISTRY II >CUSTOM<
BIOCHEMISTRY II >CUSTOM<
17th Edition
ISBN: 9781337449014
Author: GARRETT
Publisher: CENGAGE C
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Chapter 5, Problem 7P

Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.

Solving the Sequence of an Oligopeptide From Sequence AnalysisData An octapcptide consisting of 2 Gly, 1 Lys, 1 Met, I Pro, I Arg,

1 Trp, and 1 Tyr was subjected to sequence studies. The following was found:

  1. Edman degradation yielded
  2. Chapter 5, Problem 7P, Answers to all problems are at the end of this book. Detailed solutions are available in the Student

  3. Upon treatment with carboxypeptidases A. B, and Y, only carboxypeptidase Y had any effect.
  4. Trypsin treatment gave two tripeptides and a dipeptide.
  5. Chymotrypsin treatment gave two tripeplides and a dipeptide. Acid hydrolysis of the dipeptide yielded only Gly.
  6. Cyanogen bromide treatment yielded two tetrapeptides.
  7. Closlripain treatment gave a pentapeptide and a tripeptide. What is the amino acid sequence of this octapeptide?

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