BIOCHEMISTRY (LL)
9th Edition
ISBN: 9781337805100
Author: Campbell
Publisher: CENGAGE L
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Chapter 5, Problem 53RE
Interpretation Introduction
Interpretation:
The two principal types of mass spectrometry are to be defined.
Concept Information:
Mass spectrometry (MS) is the technique used in the separation of fragments of molecules according to the ratio of their mass (m) to charge (z), or m/z, ratio. This m/z ratio is used for getting information about the molecule’s structural and chemical properties, which then are used for identifying the molecule separated.
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Macmillan Learning
Cholesterol synthesis begins with the formation of mevalonate from acetyl CoA. This
process activates mevalonate and converts it to isopentenyl pyrophosphate.
Identify the atoms in mevalonate and isopentenyl pyrophosphate that will be labeled
from acetyl CoA labeled with 14C in the carbonyl carbon. Place 14C atoms and C atoms
to denote which carbon atoms are labeled and which are not labeled.
H₂C
COA
14C-labeled acetyl-CoA
HHH
[c]
H
H
OH
014C
- OH
H
HH
H
Mevalonate
CH3
H H
14C
H
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H
H
Incorrect Answer
of o
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Isopentenyl pyrophosphate
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Answer Bank
14C
Chapter 5 Solutions
BIOCHEMISTRY (LL)
Ch. 5 - RECALL What types of homogenization techniques are...Ch. 5 - RECALL When would you choose to use a...Ch. 5 - RECALL What is meant by salting out? How does it...Ch. 5 - RECALL What differences between proteins are...Ch. 5 - RECALL How could you isolate mitochondria from...Ch. 5 - RECALL Can you separate mitochondria from...Ch. 5 - RECALL Give an example of a scenario in which you...Ch. 5 - Prob. 8RECh. 5 - REFLECT AND APPLY You are purifying a protein for...Ch. 5 - Prob. 10RE
Ch. 5 - RECALL What is the basis for the separation of...Ch. 5 - RECALL What is the order of elution of proteins on...Ch. 5 - RECALL What are two ways that a compound can be...Ch. 5 - Prob. 14RECh. 5 - RECALL Why do most people elute bound proteins...Ch. 5 - RECALL What are two types of compounds that make...Ch. 5 - RECALL Draw an example of a compound that would...Ch. 5 - RECALL How can gel-filtration chromatography be...Ch. 5 - REFLECT AND APPLY Sephadex G-75 has an exclusion...Ch. 5 - Prob. 20RECh. 5 - RECALL What is the main difference between reverse...Ch. 5 - RECALL How does HPLC differ from ion-exchange...Ch. 5 - REFLECT AND APPLY Design an experiment to purify...Ch. 5 - REFLECT AND APPLY Referring to Question 23, how...Ch. 5 - Prob. 25RECh. 5 - REFLECT AND APPLY You wish to separate and purify...Ch. 5 - REFLECT AND APPLY An amino acid mixture consisting...Ch. 5 - REFLECT AND APPLY An amino acid mixture consisting...Ch. 5 - REFLECT AND APPLY In reverse-phase HPLC, the...Ch. 5 - REFLECT AND APPLY Gel-filtration chromatography is...Ch. 5 - RECALL What physical parameters of a protein...Ch. 5 - RECALL What types of compounds make up the gels...Ch. 5 - RECALL Of the two principal polymers used in...Ch. 5 - RECALL What types of macromolecules are usually...Ch. 5 - RECALL If you had a mixture of proteins with...Ch. 5 - RECALL What does SDSPAGE stand for? What is the...Ch. 5 - RECALL How does the addition of sodium...Ch. 5 - RECALL Why is the order of separation based on...Ch. 5 - RECALL The accompanying figure is from an...Ch. 5 - Prob. 40RECh. 5 - Prob. 41RECh. 5 - Prob. 42RECh. 5 - Prob. 43RECh. 5 - REFLECT AND APPLY What would happen during an...Ch. 5 - REFLECT AND APPLY A sample of an unknown peptide...Ch. 5 - REFLECT AND APPLY A sample of a peptide of unknown...Ch. 5 - REFLECT AND APPLY You are in the process of...Ch. 5 - REFLECT AND APPLY You are in the process of...Ch. 5 - Prob. 49RECh. 5 - Prob. 50RECh. 5 - Prob. 51RECh. 5 - Prob. 52RECh. 5 - Prob. 53RECh. 5 - Prob. 54RECh. 5 - RECALL What is the basis for the technique called...Ch. 5 - Prob. 56RECh. 5 - Prob. 57RECh. 5 - RECALL What are the main procedures involved in a...Ch. 5 - RECALL Where did western blot get its name?Ch. 5 - Prob. 60RECh. 5 - Prob. 61RECh. 5 - Prob. 62RECh. 5 - Prob. 63RECh. 5 - Prob. 64RECh. 5 - Prob. 65RE
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