Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 4, Problem 4Q
Summary Introduction
To analyze:
The reason for cooling the loop before touching it to culture. Whether the loop is set down for letting it cool and how to determine that it is cool.
Introduction:
An inoculating loop or just loop is a tool that is used to pick and transfer microorganisms from a culture to a new fresh growth medium. The loops used for one time only are the disposable loops made up of plastics whereas the ones made up of nichrome may be used multiple times by sterilizing them every time.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Which of the following is TRUE about immunofluorescence microscopy where we
localize proteins using fluorescently tagged antibodies? Pick the best answer:
We can use it to resolve 2 points that are 10 nanometers apart.
The emission wavelength of the fluorophore is typically shorter than the excitation
wavelength.
The resolution can be further improved with increased magnification.
All else being equal, a fluorophore that emits red light will give better resolution than a
fluorophore that emits green light.
Based on what we discussed in class, we can use the technique on live samples therefore
enabling us to see the dynamic movement of cytosolic proteins in living cells.
None of the above
The benefits of the negative stain include:
Mark all that apply:
O No shrinkage or distortion of cells due to no heat or
methanol fixing
Because the cells don't take up stain they don't get
shrunk or distorted
O One can more accurately determine cell size and shape
because the cells stand out against the background
One can more accurately determine the cell size and
shape because there is no shrinkage or distortion of cells
O Negatively charged dyes are safer to work with than
positively charged dyes
IMicrosoft B
11:32 A
You and a partner conduct the acid-fast staining using the same two bacteria. You know that there will be an acid-fast bacteria present. Your slide shows acid-fast and non-acid fast bacteria. Your partners show both bacteria are non-acid fast. Your partner doesn’t understand why. What errors have occurred?
Chapter 4 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 4 - What other methods can be used to determine...Ch. 4 - What is the primary use of slants? Of deeps?...Ch. 4 - What is the purpose of heating the loop before...Ch. 4 - Prob. 4QCh. 4 - Why is aseptic technique important?Ch. 4 - Why was the arrangement of Lactococcus in the...Ch. 4 - For a bacterium, what evolutionary advantage is...Ch. 4 - How can you tell that the media provided for this...Ch. 4 - Your microbiology lab maintains reference...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: O So that cells are not clumped and don't entrap stain creating erroneus results So that no contaminants are introduced onto the slide by being entrapped in clumps OSo that the cells are spread out enough that cell morphology can be discerned OSo that the cells are spread out enough that the arrangement can be observed O So that there are small groups of cells clumped together to make them visible Microsoft Bing 11:31 AM 87°F Sunny 9/14/2021arrow_forwardWhat is the use of a “ladder” on a gel electrophoresis?arrow_forwardHow does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: O Water must be added to a smear from liquid media but not from solid O Water must be added to a smear made from solid media but not liquid media A smear made from liquid media will be more thick than a smear made from solid O A smear made from liquid media will be thinner than a smear made from solid media O A smear made from liquid media doesn't have to be fixed but a smear from solid media does Microsoft Bing 11:31 AM 87°F Sunny 9/14/2021arrow_forward
- Why is a metal loop heat to read before picking up a bacterial sample? What should you do with a metal loop immediately after inoculating a liquid culture? How about immediately after inoculating a plate?arrow_forwardWhen preparing a "wet mount" slide of your own cheek cells, what is the name of the wet medium that will be placed over the cheek cells so that you can see them? Hypertonic saline solution Red food coloring Water Methylene blue stainarrow_forwarda. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?arrow_forward
- You and a partner conduct the acid-fast staining using the same two bacteria. You know that there will be an acid-fast bacteria present. Your slide shows acid-fast and non-acid fast bacteria. Your partners show both bacteria are non-acid fast. Your partner doesn’t understand why. What errors do you think occurred? Please state 2 and explain why these errors would cause this result.arrow_forwardIn Figure 5-3, if the concentration of bacterial cells inthe original suspension is 200/ml and 0.2 ml is platedonto each of 100 petri dishes, what is the expected average number of colonies per plate?arrow_forwardhello, can you draw the teeth smear with methylene blue on positive simple staining in 1000x objective? thank youarrow_forward
- If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? No; the clumps will wash away later. Yes; the clumps will hide the individual bacteria. The clumps will not stain. Yes; the clumps of bacterial cells may retain the stains and lead to incorrect data being collected. SubmitRequest Answerarrow_forwardWhat about the negative stain positive and negative results?arrow_forwardWhy is it essential that smears be air-dried? Why can’t they be gently heated over a flame to speed up the drying process?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education