Pathophysiology
Pathophysiology
6th Edition
ISBN: 9780323354813
Author: Jacquelyn L. Banasik PhD ARNP
Publisher: Saunders
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Chapter 4, Problem 4KQ
Summary Introduction

Damage to the cell is referred to as cellular injury. It is manifested as morphological and functional changes toward stress. Stress occurs due to internal and external environmental changes. The damage could be reversible or irreversible and it could be due to chemical, physical, and biological factors.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
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