BIOCHEMISTRY-ACHIEVE (1 TERM)
BIOCHEMISTRY-ACHIEVE (1 TERM)
9th Edition
ISBN: 9781319402853
Author: BERG
Publisher: MAC HIGHER
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Chapter 4, Problem 22P
Interpretation Introduction

Interpretation:

The primer that can be used when a reverse transcriptase activity is supposed to be assayed where polyriboadenylate is in the template needs to be determined. Also, the radioactive nucleotide that is to be used to follow chain elongation needs to be determined.

Concept introduction:

Primer is a single strand of nucleic acid. It is utilized by all the living organisms for the synthesis of DNA. The primers are removed before completion of DNA replication and DNA polymerases fill the gaps in the sequence with DNA. Scientists in the laboratory can design and synthesize DNA primers with particular sequences in a single-stranded DNA molecule that bind to DNA.

In order to perform the polymerase chain reaction, these DNA primres are commonly used to copy pieces. Shellac primers based on oil, latex and pigmented are three different types of primers

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Two tetrapeptides were isolated from a possum's sweat glands. These peptides were sequenced using Edman degradation and the following 2 sequences were obtained: Gly-Asp-Ala-Leu  Gly-Asp-Asp-Leu  Can you please help show the titration curve for both of these peptides and calculate the PI?
Two tetrapeptides were isolated from a possum's sweat glands. These peptides were sequenced using Edman degradation and the following 2 sequences were obtained: Gly-Asp-Ala-Leu  Gly-Asp-Asp-Leu  What is the structure of the PTH derivative produced during the last round of amino acid sequencing?
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