Nester's Microbiology: A Human Perspective
8th Edition
ISBN: 9780073522593
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 4, Problem 1MC
Summary Introduction
Introduction:
Generation time is an important phenomenon to be studied in population biology. An average time taken between two consecutive generations in the lineages of a population is defined as generation time. It can predict the number of population after a particular time interval.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 4 Solutions
Nester's Microbiology: A Human Perspective
Ch. 4 - Describe a detrimental and a beneficial effect of...Ch. 4 - Define a pure culture.Ch. 4 - Prob. 3SACh. 4 - Explain how the environment of a colony differs...Ch. 4 - Prob. 5SACh. 4 - Why would botulism be a concern with canned foods?Ch. 4 - Explain why O2-containing atmospheres kill some...Ch. 4 - Explain why photoautotrophs are primary producers.Ch. 4 - Distinguish between a selective medium and a...Ch. 4 - Prob. 10SA
Ch. 4 - Prob. 1MCCh. 4 - Compared with their growth in the laboratory,...Ch. 4 -
3. Cells are most sensitive to penicillin during...Ch. 4 - Which best describes the intestinal tract? a) An...Ch. 4 - E. coli, a facultative anaerobe, is grown for 24...Ch. 4 - The generation time of a bacterium was measured at...Ch. 4 - Which of the following is false? a)E. coli grows...Ch. 4 -
8. If the pH indicator were left out of MacConkey...Ch. 4 -
9. A soil sample is placed in liquid and the...Ch. 4 -
10. If the concentration of E. coli in a broth is...Ch. 4 -
1. You are a microbiologist working for a...Ch. 4 - High-performance boat manufacturers know that...Ch. 4 - This figure shows a growth curve plotted on a...Ch. 4 - Prob. 2CT
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- With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forward
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