Microbiology: A Laboratory Manual (11th Edition)
11th Edition
ISBN: 9780134098630
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Question
Chapter 4, Problem 19E.4RQ
Summary Introduction
To explain: Whether the generation time is a useful parameter to indicate the types of media best suited to support the growth of a specific organism.
Introduction: Microorganisms like bacteria are known to be important in many aspects like its application in industries that produce insulin. The study of such microorganisms helps human in many ways. To study those organisms, they should be cultivated in research laboratories with suitable culture techniques.
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You’re curious about the effect that gap genes have on the pair-rule gene, evenskipped (eve), so you isolate and sequence each of the eve enhancers. You’re particularly interested in one of the enhancers, which is just upstream of the eve gene.
Describe an experimental technique you would use to find out where this particular eve enhancer is active.
For short answer
questions, write your
answers on the line
provided. To the right is
the mRNA codon table
to use as needed
throughout the exam.
First letter
U
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U CA
UUCPhe
UUA
UCU
Phe UCC
UUG Leu
CUU
UAU.
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U
UAC TV UGCys
UAA Stop UGA Stop A
UAG Stop UGG Trp
Ser
UCA
UCG
CCU]
0
CUC
CUA
CCC
CAC
CAU His
CGU
CGC
Leu
Pro
CCA
CAA
Gin
CGA Arg
CUG
CCG
CAG
CGG
AUU
ACU
AAU T
AUC lle
A
1
ACC
Thr
AUA
ACA
AUG
Mot ACG
AGG Arg
GUU
GCU
GUC
GCC
G
Val
Ala
GAC Asp
GGU
GGC
GUA
GUG
GCA
GCG
GAA
GGA
Gly
Glu
GAGJ
GGG
AACASH AGU Ser
AAA1
AAG Lys
GAU
AGA
CAL CALUCAO CAO
G
Third letter
1. (+7) Use the table below to answer the questions; use the codon table above to assist you.
The promoter sequence of DNA is on the LEFT. You do not need to fill in the entire table.
Assume we are in the middle of a gene sequence (no need to find a start codon).
DNA
1
DNA
2
mRNA
tRNA
Polypeptide
C
Val
G
C.
T
A
C
a. On which strand of DNA is the template strand (DNA 1 or 2)?_
b. On which side of the mRNA is the 5' end (left or…
3. (6 pts) Fill in the boxes according to the directions on the right.
Structure
R-C
R-COOH
OH
R-OH
i
R-CO-R' R
R-PO4
R-CH3
C.
0
R'
R-O-P-OH
1
OH
H
R-C-H
R-N'
I-
H
H
R-NH₂
\H
Name
Properties
Chapter 4 Solutions
Microbiology: A Laboratory Manual (11th Edition)
Ch. 4 - Prob. 12E.1RQCh. 4 - Prob. 12E.2RQCh. 4 - Prob. 12E.3RQCh. 4 - Prob. 12E.4RQCh. 4 - A soil isolate is found to grow poorly in a basic...Ch. 4 - Prob. 13E.1RQCh. 4 - Why are crystal violet agar and 7.5% sodium...Ch. 4 - A patient exhibits a boil on his neck. You, as a...Ch. 4 - In the following chart, indicate the types of...Ch. 4 - Explain the effects of temperatures above the...
Ch. 4 - Prob. 14E.3RQCh. 4 - Is it possible for thermophilic organisms to...Ch. 4 - Prob. 15E.1RQCh. 4 - Prob. 15E.2RQCh. 4 - Prob. 15E.3RQCh. 4 - Prob. 15E.4RQCh. 4 - Prob. 15E.5RQCh. 4 - Prob. 15E.6RQCh. 4 - Why is it necessary to place the inoculated molten...Ch. 4 - Prob. 16E.2RQCh. 4 - Prob. 16E.3RQCh. 4 - Prob. 16E.4RQCh. 4 - Prob. 16E.5RQCh. 4 - Prob. 16E.6RQCh. 4 - Prob. 16E.7RQCh. 4 - Prob. 17E.1RQCh. 4 - Prob. 17E.2RQCh. 4 - Prob. 17E.3RQCh. 4 - Prob. 17E.4RQCh. 4 - The physician who treats your puncture wound opts...Ch. 4 - Prob. 18E.1RQCh. 4 - Prob. 18E.2RQCh. 4 - Prob. 18E.3RQCh. 4 - Prob. 18E.4RQCh. 4 - Prob. 18E.5RQCh. 4 - Prob. 18E.6RQCh. 4 - Prob. 18E.7RQCh. 4 - Prob. 19E.1RQCh. 4 - Why do variations in generation time exist
Among...Ch. 4 - Prob. 19E.3RQCh. 4 - Prob. 19E.4RQ
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