Prescott's Microbiology
Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 37.3, Problem 4CC
Summary Introduction

To explain: The indirect ELISA technique.

Introduction: ELISA or enzyme-linked immunosorbent assay is a biochemical diagnostic assay used to measure the concentration of antibody or antigen in the test blood sample.

Summary Introduction

To explain: The direct ELISA technique.

Introduction: The principle of ELISA involves the detection of the Ag-Ab complex with the help of an enzyme that binds to this complex and carries out the conversion of a colorless substrate to a colored product. This indicates the presence of the Ag-Ab complex in the sample.

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