<LCPO> BIOLOGY
12th Edition
ISBN: 9781266216398
Author: Raven
Publisher: MCG CUSTOM
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Chapter 37.3, Problem 1LO
Summary Introduction
To explain: The significance of nitrogen-fixing bacteria for plant nutrition.
Introduction: The plants are mainly multicellular, predominantly photosynthetic eukaryotes of the kingdom Plantae. The plant nutrients are defined as the chemical elements that are important for the nourishment of the health of a plant. There are two types of nutrients such as macronutrients and micronutrients that are obtained by the plant from their growing medium.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
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oxygen is terminal electron acceptor
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Chapter 37 Solutions
<LCPO> BIOLOGY
Ch. 37.1 - Prob. 1LOCh. 37.1 - Prob. 2LOCh. 37.1 - Prob. 3LOCh. 37.2 - Prob. 1LOCh. 37.2 - Prob. 2LOCh. 37.2 - Describe the goal of food fortification research.Ch. 37.3 - Prob. 1LOCh. 37.3 - Prob. 2LOCh. 37.3 - Prob. 3LOCh. 37.4 - Prob. 1LO
Ch. 37.4 - Explain the main effect on herbivores of a higher...Ch. 37.4 - Prob. 3LOCh. 37.5 - Prob. 1LOCh. 37.5 - Prob. 2LOCh. 37.5 - Prob. 3LOCh. 37 - Prob. 1IQCh. 37 - Prob. 2IQCh. 37 - Which of the following is NOT found in topsoil? a....Ch. 37 - Prob. 2UCh. 37 - What proportion of the soil volume is occupied by...Ch. 37 - Which of the following is a micronutrient? a....Ch. 37 - Prob. 5UCh. 37 - Prob. 6UCh. 37 - a C4 plant, the Calvin cycle occurs in a. the...Ch. 37 - One potential problem with using poplars to remove...Ch. 37 - Prob. 1ACh. 37 - If you wanted to conduct an experiment to...Ch. 37 - Which of the following would decrease nitrogen...Ch. 37 - Which of the following might you do to increase...Ch. 37 - Prob. 5ACh. 37 - Prob. 1SCh. 37 - Describe an experiment to determine the amount of...Ch. 37 - Prob. 3S
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