PRESCOTT'S MICROBILOGY
11th Edition
ISBN: 9781264075515
Author: WILLEY
Publisher: MCG
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Textbook Question
Chapter 37.2, Problem 2CC
Describe in general how biochemical tests are used in the API 20E system to identify bacteria.
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Why are biochemical tests used to identify microbes?
Explain with the examples of biochemical test that are used to identify the bacteria in the
laboratory.
The correct order of steps when purifying a protein are:
Group of answer choices
1)Growing bacterial culture for fusion protein; 2)Harvesting IPTG-induced cultures; 3)Lysing bacterial cell; 4)Removing insoluble debris; 5)Using Affinity Chromatography to purify protein.
1)Growing bacterial culture for fusion protein; 2) Breaking open bacterial cells; 3) Removing insoluble debris: 4) Harvesting IPTG-induced cultures; 5)Using Affinity Chromatography to purify protein
1) Growing bacterial culture for fusion protein; 2) Lysing bacterial cells; 3) Harvesting IPTG-induced cultures; 4) Removing insoluble debris: 5)Purifying protein by affinity chromatography
1)Growing bacterial culture for fusion protein; 2) Removing insoluble debris; 3) Harvesting IPTG-induced cultures; 4)Breaking open bacterial cells; 5)Purifying protein by affinity chromatography
Chapter 37 Solutions
PRESCOTT'S MICROBILOGY
Ch. 37.2 - Prob. 1MICh. 37.2 - Prob. 2MICh. 37.2 - Prob. 1CCCh. 37.2 - Describe in general how biochemical tests are used...Ch. 37.2 - Prob. 3CCCh. 37.2 - Prob. 4CCCh. 37.2 - Prob. 5CCCh. 37.2 - Prob. 6CCCh. 37.3 - Prob. 1MICh. 37.3 - Prob. 2MI
Ch. 37.3 - Prob. 1CCCh. 37.3 - Prob. 2CCCh. 37.3 - Prob. 3CCCh. 37.3 - Prob. 4CCCh. 37.3 - Prob. 5CCCh. 37.3 - Name two types of immunodiffusion tests and...Ch. 37 - Prob. 1RCCh. 37 - Prob. 2RCCh. 37 - Prob. 3RCCh. 37 - Prob. 4RCCh. 37 - ELISA tests usually use a primary and secondary...Ch. 37 - Prob. 2ALCh. 37 - Prob. 3AL
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- Biochemical tests such as the fermentation test can be used when unknown bacterial cells are identified. true or false?arrow_forwardBriefly explain how you could use the color of the Coomassie assay sample solution to determine if anunknown sample needs to be diluted in order to measure the sample’s concentrationarrow_forwardDescribe the examples of biochemical test that are used to identify the bacteria in the laboratory.arrow_forward
- Explain how are the physiological characteristics of bacteria identified using biochemical test?arrow_forwardpresent briefly the principle of size exclusion chromatography. give a brief description of the use of protein assays.arrow_forwardPlease discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.arrow_forward
- What are the disadvantages of biochemical test?arrow_forwardGive the principle involved in the ninhydrin test. What is its purpose?arrow_forwardIf a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe growing within the zone of inhibitionfor a drug, what should her conclusion be?arrow_forward
- You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture. What is the first step to prepare your Kirby-Bauer test?arrow_forwardBiochemical and differential tests can be incorporated into growth media or performed on isolated colonies after they have been cultured. Give three examples of a differential test that is incorporated into media and three examples that are applied after the colonies have been cultured.arrow_forwardBriefly compare the following test systems, Micro-tox, ATP-Tox, and Daphnia magna IQ test . Explain how each assay works (the principle), not the test procedure.arrow_forward
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