Essentials of Biology
4th Edition
ISBN: 9780078024221
Author: Sylvia S. Mader Dr., Michael Windelspecht
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 32.3, Problem 7A
Most of the fresh water in the world is used for.
- drinking.
- supporting industry.
- irrigating crops.
- cooking.
- bathing.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 32 Solutions
Essentials of Biology
Ch. 32.1 - Prob. 1LOCh. 32.1 - Prob. 2LOCh. 32.1 - Prob. 1CYPCh. 32.1 - Prob. 2CYPCh. 32.1 - Prob. 3CYPCh. 32.1 - Prob. 1ACh. 32.1 - Prob. 2ACh. 32.2 - Define biodiversity, and briefly list some of the...Ch. 32.2 - Prob. 2LOCh. 32.2 - Prob. 3LO
Ch. 32.2 - Prob. 1CYPCh. 32.2 - Prob. 2CYPCh. 32.2 - Prob. 3CYPCh. 32.2 - Prob. 3ACh. 32.2 - Prob. 4ACh. 32.3 - Describe the effects of human populations on land...Ch. 32.3 - Prob. 2LOCh. 32.3 - Prob. 3LOCh. 32.3 - Prob. 1CYPCh. 32.3 - Prob. 2CYPCh. 32.3 - 3. List the harmful effects of modern agricultural...Ch. 32.3 - Prob. 4CYPCh. 32.3 - Prob. 5CYPCh. 32.3 - Prob. 6CYPCh. 32.3 - Prob. 5ACh. 32.3 - Prob. 6ACh. 32.3 - Most of the fresh water in the world is used for....Ch. 32.3 - 8.A major negative effect of the dumping of raw...Ch. 32.3 - Prob. 9ACh. 32.3 - Prob. 10ACh. 32.4 - 1. Explain why human society is unsustainable in...Ch. 32.4 - Prob. 2LOCh. 32.4 - Prob. 3LOCh. 32.4 - Prob. 1CYPCh. 32.4 - Prob. 2CYPCh. 32.4 - Prob. 3CYPCh. 32.4 - Prob. 11ACh. 32.4 - Prob. 12ACh. 32 - Prob. S12.3BYBCh. 32 - Prob. S30.2BYBCh. 32 - Prob. S31.2BYBCh. 32 - Prob. 1BYBCh. 32 - Prob. 2BYBCh. 32 - Prob. S18.2CTCCh. 32 - Prob. S30.2CTCCh. 32 - Prob. S31.2CTCCh. 32 - In 1993, the U.S. Fish and Wildlife Service...Ch. 32 - Prob. 2TCCh. 32 - Prob. 3TC
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