2 SEM ACC W/RAVEN CARDED
12th Edition
ISBN: 9781264439218
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 32, Problem 7U
Which of the following characteristics is used to distinguish between a parazoan and a eumetazoan?
a. Presence of a true coelom
b. Segmentation
c. Cephalization
d. Tissues
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
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3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 32 Solutions
2 SEM ACC W/RAVEN CARDED
Ch. 32.1 - Prob. 1LOCh. 32.2 - Prob. 1LOCh. 32.2 - Prob. 2LOCh. 32.2 - Prob. 3LOCh. 32.3 - Prob. 1LOCh. 32.3 - Prob. 2LOCh. 32.3 - Prob. 3LOCh. 32.4 - Prob. 1LOCh. 32.4 - Prob. 2LOCh. 32.5 - Prob. 1LO
Ch. 32.5 - Prob. 2LOCh. 32.5 - Prob. 3LOCh. 32.6 - Prob. 1LOCh. 32.6 - Prob. 2LOCh. 32 - Prob. 1IQCh. 32 - Prob. 1UCh. 32 - Animals are unique in the fact that they possess...Ch. 32 - In animal sexual reproduction the gametes are...Ch. 32 - The evolution of bilateral symmetry was a...Ch. 32 - A fluid-filled cavity that develops completely...Ch. 32 - Which of the following statements is NOT true...Ch. 32 - Which of the following characteristics is used to...Ch. 32 - With regard to classification of animals, the...Ch. 32 - The coelom a. is a synapomorphy for the clade...Ch. 32 - A coelomate organism may have which of the...Ch. 32 - Which of the following cell types of a sponge...Ch. 32 - Prob. 12UCh. 32 - Prob. 13UCh. 32 - The following diagram is of the blastopore sage of...Ch. 32 - Prob. 2ACh. 32 - Prob. 3ACh. 32 - Which of the following cell layers is not...Ch. 32 - Prob. 2S
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