PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781264073375
Author: WILLEY
Publisher: MCG
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Chapter 31.4, Problem 3CC
What microbial genera have been observed in oil field materials?
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5G JA ATTC 3
3 CTIA A1G5
5 GAAT I I3
3 CTIA AA5
Fig. 5-3: The Eco restriction site (left) would be cleaved at the locations indicated by the arrows.
However, a SNP in the position shown in gray (right) would prevent cleavage at this site by EcoRI
One of the SNPs in B. rapa is found within the Park14 locus and can be detected
by RFLP analysis. The CT polymorphism is found in the intron of the Bra013780
gene found on Chromosome 1. The Park14 allele with the "C" in the SNP has two
EcoRI sites and thus is cleaved twice by EcoRI If there is a "T" in that SNP, one
of the EcoRI sites is altered, so the Park14 allele with the T in the SNP has only
one EcoRI site (Fig. 5-3).
Park14 allele
with SNP(C)
Park14 allele
with SNPT)
839 EcoRI
1101 EcoRI
839 EcoRI
Fig. 5.4: Schematic restriction maps of the two different Park14 alleles (1316 bp long) of B. rapa. Where
on these maps is the CT SNP located?
90
The primers used to amplify the DNA at the Park14 locus (see Fig. 5 and Table 3
of Slankster et…
Chapter 31 Solutions
PRESCOTT'S MICROBIOLOGY
Ch. 31.1 - How are filamentous microbes especially well...Ch. 31.1 - Prob. 1CCCh. 31.1 - Prob. 2CCCh. 31.1 - What microbes commonly degrade cellulose and...Ch. 31.1 - Which of the following soils would a farmer be...Ch. 31.1 - Why is most nitrogen fertilizer added as ammonium...Ch. 31.2 - What are the differences in preferred soil...Ch. 31.2 - What types of archaea have been detected in soils?Ch. 31.2 - Prob. 3CCCh. 31.3 - Define rhizosphere, rhizoplane, and associative...
Ch. 31.3 - What unique stresses does a microorganism on a...Ch. 31.3 - List two ways in which compounds produced by...Ch. 31.3 - What important genera are involved in associative...Ch. 31.3 - Prob. 1MICh. 31.3 - Prob. 2.1CCCh. 31.3 - Prob. 2.2CCCh. 31.3 - What is the function of the rhizomorph and the...Ch. 31.3 - Describe the uptake and transfer of ammonium by...Ch. 31.3 - Propose two potential functions for mycorrhization...Ch. 31.3 - How do you think Frankia spp. protect nitrogenase...Ch. 31.3 - List several bacteria that are considered...Ch. 31.3 - Prob. 3.2CCCh. 31.3 - What does the term terminally differentiated mean?Ch. 31.3 - How does nitrogen transfer between a rhizobium and...Ch. 31.3 - What is unusual about leghemoglobin production and...Ch. 31.3 - What are the two general mechanisms by which...Ch. 31.3 - How does the production of opines by the plant...Ch. 31.3 - What is the difference between the Ti plasmid and...Ch. 31.3 - What functions do the members of the two-component...Ch. 31.3 - What are the two general ways by which plant...Ch. 31.3 - How are plant pathologists attempting to control...Ch. 31.4 - Compare and contrast the metabolism (specifically,...Ch. 31.4 - What happens in terms of microbiological processes...Ch. 31.4 - What microbial genera have been observed in oil...Ch. 31 - Prob. 1RCCh. 31 - Prob. 2RCCh. 31 - Prob. 3RCCh. 31 - Prob. 4RCCh. 31 - Prob. 5RCCh. 31 - Prob. 6RCCh. 31 - Prob. 7RC
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