Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that would result from digesting pLAB30 with EcoR1, with BamHI, and with both enzymes together. Which enzyme will give the smallest piece containing the tetracycline-resistance gene?

Question

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Answer 1

Textbook Question

Chapter 30, Problem 2Q

Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that would result from digesting pLAB30 with EcoR1, with BamHI, and with both enzymes together.

Chapter 30, Problem 2Q, Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that , example 1

Chapter 30, Problem 2Q, Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that , example 2

Which enzyme will give the smallest piece containing the tetracycline-resistance gene?

Expert Solution & Answer

Summary Introduction

To determine:

The size and number of DNA Fragments given by restriction enzymes Eco RI, Bam HI, and both these enzymes together.

Introduction:

Restriction enzyme is an enzyme that cuts the DNA fragment at the specific site by recognizing the DNA sequence at that site. The site at which the DNA is cut is termed as the restriction site.

Explanation of Solution

pLAB30 is an 18 kb map, which is cut by two restriction enzymes, Eco RI, and Bam HI. When Eco RI acts, then it cuts the DNA fragment at two sites, giving an 11 kb fragment and 7 kb fragment of the DNA.

When Bam HIacts, then it cuts DNA fragment at three sites, giving three fragments. The size of these fragments is 9 kb, 3 kb, and 6 kb.

When both these restriction enzymes act together, then 5 DNA fragments are obtained. The size of these fragments is 1 kb, 3 kb, 3 kb, 3kb, and 8 kb.

Conclusion

The table showing the size and number of DNA fragments after the DNA is acted upon by restriction enzymes is shown below:

Restriction enzyme	Number of DNA fragments obtained	Size of DNA fragments
Eco RI	2	11 kb and 7 kb
Bam HI	3	9 kb, 3 kb, and 6 kb
Eco RI + Bam HI	5	1 kb, 3 kb, 3 kb, 3kb, and 8 kb

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Answer 2

Textbook Question

Chapter 30, Problem 2Q

Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that would result from digesting pLAB30 with EcoR1, with BamHI, and with both enzymes together.

Chapter 30, Problem 2Q, Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that , example 1

Chapter 30, Problem 2Q, Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that , example 2

Which enzyme will give the smallest piece containing the tetracycline-resistance gene?

Expert Solution & Answer

Summary Introduction

To determine:

The size and number of DNA Fragments given by restriction enzymes Eco RI, Bam HI, and both these enzymes together.

Introduction:

Restriction enzyme is an enzyme that cuts the DNA fragment at the specific site by recognizing the DNA sequence at that site. The site at which the DNA is cut is termed as the restriction site.

Explanation of Solution

pLAB30 is an 18 kb map, which is cut by two restriction enzymes, Eco RI, and Bam HI. When Eco RI acts, then it cuts the DNA fragment at two sites, giving an 11 kb fragment and 7 kb fragment of the DNA.

When Bam HIacts, then it cuts DNA fragment at three sites, giving three fragments. The size of these fragments is 9 kb, 3 kb, and 6 kb.

When both these restriction enzymes act together, then 5 DNA fragments are obtained. The size of these fragments is 1 kb, 3 kb, 3 kb, 3kb, and 8 kb.

Conclusion

The table showing the size and number of DNA fragments after the DNA is acted upon by restriction enzymes is shown below:

Restriction enzyme	Number of DNA fragments obtained	Size of DNA fragments
Eco RI	2	11 kb and 7 kb
Bam HI	3	9 kb, 3 kb, and 6 kb
Eco RI + Bam HI	5	1 kb, 3 kb, 3 kb, 3kb, and 8 kb

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Answer 3

Textbook Question

Chapter 30, Problem 2Q

Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that would result from digesting pLAB30 with EcoR1, with BamHI, and with both enzymes together.

Chapter 30, Problem 2Q, Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that , example 1

Chapter 30, Problem 2Q, Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that , example 2

Which enzyme will give the smallest piece containing the tetracycline-resistance gene?

Expert Solution & Answer

Summary Introduction

To determine:

The size and number of DNA Fragments given by restriction enzymes Eco RI, Bam HI, and both these enzymes together.

Introduction:

Restriction enzyme is an enzyme that cuts the DNA fragment at the specific site by recognizing the DNA sequence at that site. The site at which the DNA is cut is termed as the restriction site.

Explanation of Solution

pLAB30 is an 18 kb map, which is cut by two restriction enzymes, Eco RI, and Bam HI. When Eco RI acts, then it cuts the DNA fragment at two sites, giving an 11 kb fragment and 7 kb fragment of the DNA.

When Bam HIacts, then it cuts DNA fragment at three sites, giving three fragments. The size of these fragments is 9 kb, 3 kb, and 6 kb.

When both these restriction enzymes act together, then 5 DNA fragments are obtained. The size of these fragments is 1 kb, 3 kb, 3 kb, 3kb, and 8 kb.

Conclusion

The table showing the size and number of DNA fragments after the DNA is acted upon by restriction enzymes is shown below:

Restriction enzyme	Number of DNA fragments obtained	Size of DNA fragments
Eco RI	2	11 kb and 7 kb
Bam HI	3	9 kb, 3 kb, and 6 kb
Eco RI + Bam HI	5	1 kb, 3 kb, 3 kb, 3kb, and 8 kb

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Answer 4

Textbook Question

Chapter 30, Problem 2Q

Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that would result from digesting pLAB30 with EcoR1, with BamHI, and with both enzymes together.

Chapter 30, Problem 2Q, Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that , example 1

Chapter 30, Problem 2Q, Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that , example 2

Which enzyme will give the smallest piece containing the tetracycline-resistance gene?

Expert Solution & Answer

Summary Introduction

To determine:

The size and number of DNA Fragments given by restriction enzymes Eco RI, Bam HI, and both these enzymes together.

Introduction:

Restriction enzyme is an enzyme that cuts the DNA fragment at the specific site by recognizing the DNA sequence at that site. The site at which the DNA is cut is termed as the restriction site.

Explanation of Solution

pLAB30 is an 18 kb map, which is cut by two restriction enzymes, Eco RI, and Bam HI. When Eco RI acts, then it cuts the DNA fragment at two sites, giving an 11 kb fragment and 7 kb fragment of the DNA.

When Bam HIacts, then it cuts DNA fragment at three sites, giving three fragments. The size of these fragments is 9 kb, 3 kb, and 6 kb.

When both these restriction enzymes act together, then 5 DNA fragments are obtained. The size of these fragments is 1 kb, 3 kb, 3 kb, 3kb, and 8 kb.

Conclusion

The table showing the size and number of DNA fragments after the DNA is acted upon by restriction enzymes is shown below:

Restriction enzyme	Number of DNA fragments obtained	Size of DNA fragments
Eco RI	2	11 kb and 7 kb
Bam HI	3	9 kb, 3 kb, and 6 kb
Eco RI + Bam HI	5	1 kb, 3 kb, 3 kb, 3kb, and 8 kb

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Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

To determine:

The size and number of DNA Fragments given by restriction enzymes Eco RI, Bam HI, and both these enzymes together.

Introduction:

Restriction enzyme is an enzyme that cuts the DNA fragment at the specific site by recognizing the DNA sequence at that site. The site at which the DNA is cut is termed as the restriction site.

Explanation of Solution

pLAB30 is an 18 kb map, which is cut by two restriction enzymes, Eco RI, and Bam HI. When Eco RI acts, then it cuts the DNA fragment at two sites, giving an 11 kb fragment and 7 kb fragment of the DNA.

When Bam HIacts, then it cuts DNA fragment at three sites, giving three fragments. The size of these fragments is 9 kb, 3 kb, and 6 kb.

When both these restriction enzymes act together, then 5 DNA fragments are obtained. The size of these fragments is 1 kb, 3 kb, 3 kb, 3kb, and 8 kb.

Conclusion

The table showing the size and number of DNA fragments after the DNA is acted upon by restriction enzymes is shown below:

Restriction enzyme	Number of DNA fragments obtained	Size of DNA fragments
Eco RI	2	11 kb and 7 kb
Bam HI	3	9 kb, 3 kb, and 6 kb
Eco RI + Bam HI	5	1 kb, 3 kb, 3 kb, 3kb, and 8 kb

Answer 8

Expert Solution & Answer

Summary Introduction

To determine:

The size and number of DNA Fragments given by restriction enzymes Eco RI, Bam HI, and both these enzymes together.

Introduction:

Restriction enzyme is an enzyme that cuts the DNA fragment at the specific site by recognizing the DNA sequence at that site. The site at which the DNA is cut is termed as the restriction site.

Explanation of Solution

pLAB30 is an 18 kb map, which is cut by two restriction enzymes, Eco RI, and Bam HI. When Eco RI acts, then it cuts the DNA fragment at two sites, giving an 11 kb fragment and 7 kb fragment of the DNA.

When Bam HIacts, then it cuts DNA fragment at three sites, giving three fragments. The size of these fragments is 9 kb, 3 kb, and 6 kb.

When both these restriction enzymes act together, then 5 DNA fragments are obtained. The size of these fragments is 1 kb, 3 kb, 3 kb, 3kb, and 8 kb.

Conclusion

The table showing the size and number of DNA fragments after the DNA is acted upon by restriction enzymes is shown below:

Restriction enzyme	Number of DNA fragments obtained	Size of DNA fragments
Eco RI	2	11 kb and 7 kb
Bam HI	3	9 kb, 3 kb, and 6 kb
Eco RI + Bam HI	5	1 kb, 3 kb, 3 kb, 3kb, and 8 kb

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

To determine:

The size and number of DNA Fragments given by restriction enzymes Eco RI, Bam HI, and both these enzymes together.

Introduction:

Restriction enzyme is an enzyme that cuts the DNA fragment at the specific site by recognizing the DNA sequence at that site. The site at which the DNA is cut is termed as the restriction site.

Answer 12

Explanation of Solution

pLAB30 is an 18 kb map, which is cut by two restriction enzymes, Eco RI, and Bam HI. When Eco RI acts, then it cuts the DNA fragment at two sites, giving an 11 kb fragment and 7 kb fragment of the DNA.

When Bam HIacts, then it cuts DNA fragment at three sites, giving three fragments. The size of these fragments is 9 kb, 3 kb, and 6 kb.

When both these restriction enzymes act together, then 5 DNA fragments are obtained. The size of these fragments is 1 kb, 3 kb, 3 kb, 3kb, and 8 kb.

Answer 13

Conclusion

The table showing the size and number of DNA fragments after the DNA is acted upon by restriction enzymes is shown below:

Restriction enzyme	Number of DNA fragments obtained	Size of DNA fragments
Eco RI	2	11 kb and 7 kb
Bam HI	3	9 kb, 3 kb, and 6 kb
Eco RI + Bam HI	5	1 kb, 3 kb, 3 kb, 3kb, and 8 kb

Answer 14

Ch. 30 - Using this map of pLAB30 (18 kb), give the number...Ch. 30 - Differentiate a gene from a RFLP.Ch. 30 - Prob. 2CT Ch. 30 - Prob. 2CA

Using this map of pLAB30 (18 kb), give the number and lengths of the restriction fragments that would result from digesting pLAB30 with EcoR1, with BamHI, and with both enzymes together. Which enzyme will give the smallest piece containing the tetracycline-resistance gene?

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