Connect With Learnsmart Labs Online Access For Prescott's Microbiology
Connect With Learnsmart Labs Online Access For Prescott's Microbiology
11th Edition
ISBN: 9781260408997
Author: Joanne Willey
Publisher: Mcgraw-hill Higher Education (us)
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Chapter 30, Problem 1RC
Summary Introduction

To determine: The idea about the global impact that microbes possess on carbon and nitrogen cycling

Introduction: Carbon and nitrogen cycle are the pathways, in which carbon and nitrogen gas become available to the organisms, through the various means. The gases after use convert back into the original form helped by several factors so that the regulation of gases can be maintained.

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Explanation of Solution

Microbes are the key factors regulating various types of gases in the environment, which these microbes utilize, and for the utilization purpose, these microbes break down the complex organic compounds into the simpler and consumable forms. Such as nitrogen remains present in the atmosphere in the form of molecular nitrogen, which cannot be available to the plants. However, the root living microbes which survive on the leguminous plant roots fix the nitrogen in the form of nitrates, which is absorbed by the plants.

Similarly, carbon dioxide is an essentialgas of the atmospheric as well as greenhouse gases. The overmisuse of carbon dioxide and the industrial revolution have made carbon flux to be increased uptoten folds by annual. However, the microbes play a role in fixing the atmospheric CO2 by involving in the global carbon cycle to sequester the management of carbon emission. The mostcommon carbon fixing microbes are phototrophic and autotrophic.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin

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Connect With Learnsmart Labs Online Access For Prescott's Microbiology

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