Biology Now (Second Edition)
2nd Edition
ISBN: 9780393631807
Author: Anne Houtman, Megan Scudellari, Cindy Malone
Publisher: W. W. Norton & Company
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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important!
3’- T A T C C G A C A A T C G A T C G A T T G C C T T C T A A -5’
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