Human Anatomy
6th Edition
ISBN: 9781260399820
Author: SALADIN, Kenneth
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 3, Problem 3.6.4AYLO
The meaning of tissue atrophy, its causes, and examples
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a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Chapter 3 Solutions
Human Anatomy
Ch. 3.1 - Define tissue and distinguish a tissue from a cell...Ch. 3.1 - Prob. 2BYGOCh. 3.1 - Prob. 3BYGOCh. 3.1 - Prob. 4BYGOCh. 3.1 - Prob. 5BYGOCh. 3.2 - Distinguish between simple and stratified...Ch. 3.2 - Prob. 7BYGOCh. 3.2 - Prob. 8BYGOCh. 3.2 - Prob. 9BYGOCh. 3.2 - Prob. 10BYGO
Ch. 3.2 - Explain how urothelium is specifically adapted to...Ch. 3.3 - When the following tissues are injured, which do...Ch. 3.3 - Prob. 12BYGOCh. 3.3 - Prob. 13BYGOCh. 3.3 - Prob. 14BYGOCh. 3.3 - Prob. 15BYGOCh. 3.3 - Discuss the difference between dense regular and...Ch. 3.3 - Describe some similarities, differences, and...Ch. 3.3 - What are the three basic kinds of formed elements...Ch. 3.4 - Although the nuclei of a muscle fiber are pressed...Ch. 3.4 - What do nervous muscular tissue have in common?...Ch. 3.4 - What are the two basic types of cells in nervous...Ch. 3.4 - Name the three kinds of muscular tissue, describe...Ch. 3.5 - Distinguish between a simple gland and a compound...Ch. 3.5 - Prob. 23BYGOCh. 3.5 - Prob. 24BYGOCh. 3.5 - Prob. 25BYGOCh. 3.6 - What functions of a ciliated pseudostratified...Ch. 3.6 - Tissues can grow through an increase in cell size...Ch. 3.6 - Distinguish between differentiation and...Ch. 3.6 - Distinguish between regeneration and fibrosis....Ch. 3.6 - Prob. 29BYGOCh. 3 - Prob. 3.1.1AYLOCh. 3 - Prob. 3.1.2AYLOCh. 3 - Prob. 3.1.3AYLOCh. 3 - Prob. 3.1.4AYLOCh. 3 - Prob. 3.1.5AYLOCh. 3 - Prob. 3.1.6AYLOCh. 3 - Prob. 3.1.7AYLOCh. 3 - Prob. 3.2.1AYLOCh. 3 - The location, composition, and functions of a...Ch. 3 - Prob. 3.2.3AYLOCh. 3 - Prob. 3.2.4AYLOCh. 3 - The appearance, representative locations, and...Ch. 3 - Prob. 3.2.6AYLOCh. 3 - Differences in structure, location, and function...Ch. 3 - The process of exfoliation and a clinical...Ch. 3 - Prob. 3.3.1AYLOCh. 3 - Prob. 3.3.2AYLOCh. 3 - The types of connective tissue classified as...Ch. 3 - Prob. 3.3.4AYLOCh. 3 - The distinction between loose and dense fibrous...Ch. 3 - The appearance, representative locations, and...Ch. 3 - The appearance, representative locations, and...Ch. 3 - Prob. 3.3.8AYLOCh. 3 - Prob. 3.3.9AYLOCh. 3 - The relationship of the perichondrium to...Ch. 3 - Prob. 3.3.11AYLOCh. 3 - Prob. 3.3.12AYLOCh. 3 - Prob. 3.3.13AYLOCh. 3 - Prob. 3.3.14AYLOCh. 3 - Prob. 3.3.15AYLOCh. 3 - Why blood is considered a connective tissueCh. 3 - Prob. 3.3.17AYLOCh. 3 - Prob. 3.3.18AYLOCh. 3 - The meaning of cell excitability, and why nervous...Ch. 3 - Prob. 3.4.2AYLOCh. 3 - Prob. 3.4.3AYLOCh. 3 - Prob. 3.4.4AYLOCh. 3 - The defining characteristics of muscular tissue as...Ch. 3 - Prob. 3.4.6AYLOCh. 3 - Prob. 3.4.7AYLOCh. 3 - The microscopio representative locations, and...Ch. 3 - Prob. 3.5.1AYLOCh. 3 - The distinction between exocrine and eadocrine...Ch. 3 - Prob. 3.5.3AYLOCh. 3 - Prob. 3.5.4AYLOCh. 3 - Prob. 3.5.5AYLOCh. 3 - Prob. 3.5.6AYLOCh. 3 - The distinctions between eccrine, apocrine, and...Ch. 3 - Prob. 3.5.8AYLOCh. 3 - The tissue layers of a mucous membrane and of a...Ch. 3 - The nature and locations of endothelium,...Ch. 3 - Prob. 3.6.1AYLOCh. 3 - The difference between differentiation and...Ch. 3 - Two ways in which the body repairs damaged...Ch. 3 - The meaning of tissue atrophy, its causes, and...Ch. 3 - Prob. 3.6.5AYLOCh. 3 - Prob. 3.6.6AYLOCh. 3 - Prob. 1TYRCh. 3 - Prob. 2TYRCh. 3 - Prob. 3TYRCh. 3 - A seminiferous tubule of the testis is lined with...Ch. 3 - Prob. 5TYRCh. 3 - Prob. 6TYRCh. 3 - Prob. 7TYRCh. 3 - Tendons are composed of _________ connective...Ch. 3 - The shape of the external ear is due to skeletan...Ch. 3 - The most abundant formed elements(s) of blood...Ch. 3 - Prob. 11TYRCh. 3 - Prob. 12TYRCh. 3 - Prob. 13TYRCh. 3 - Prob. 14TYRCh. 3 - Tendons and ligaments are made mainly of the...Ch. 3 - Prob. 16TYRCh. 3 - Prob. 17TYRCh. 3 - Prob. 18TYRCh. 3 - Prob. 19TYRCh. 3 - Prob. 20TYRCh. 3 - Prob. 1BYMVCh. 3 - Prob. 2BYMVCh. 3 - Prob. 3BYMVCh. 3 - Prob. 4BYMVCh. 3 - Prob. 5BYMVCh. 3 - State a meaning of each word element and give a...Ch. 3 - State a meaning of each word element and give a...Ch. 3 - State a meaning of each word element and give a...Ch. 3 - Prob. 9BYMVCh. 3 - Prob. 10BYMVCh. 3 - Prob. 1WWWTSCh. 3 - Prob. 2WWWTSCh. 3 - Prob. 3WWWTSCh. 3 - Prob. 4WWWTSCh. 3 - Prob. 5WWWTSCh. 3 - Prob. 6WWWTSCh. 3 - Prob. 7WWWTSCh. 3 - Prob. 8WWWTSCh. 3 - Prob. 9WWWTSCh. 3 - Prob. 10WWWTSCh. 3 - Prob. 1TYCCh. 3 - Prob. 2TYCCh. 3 - Prob. 3TYCCh. 3 - Prob. 4TYCCh. 3 - Some human cells are incapable of mitosis...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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