BIOL 105 LIVING WORLD CONNECT CARD
BIOL 105 LIVING WORLD CONNECT CARD
9th Edition
ISBN: 9781260419979
Author: Johnson
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 3, Problem 2IA

a.

Summary Introduction

To determine: The percentage of hemoglobin bound to oxygen at all the three pH concentrations during saturation and at the different oxygen levels such as 20mm Hg, 40mm Hg, and 60mm Hg.

Introduction: Red blood cells are formed in the spleen and bone marrow. These cells have a life span of about 120 days. The color of red blood cells is red due to the presence of a protein called hemoglobin. This protein carries oxygen to all body parts.

b.

Summary Introduction

To determine: The effect of oxygen level in the blood on the binding of oxygen to hemoglobin.

Introduction: Oxygen is essential for different processes in the body. It is necessary for respiration, repairing body cells, feeding nerve cells, and cleansing body tissues.

c.

Summary Introduction

To determine:  The significant difference between the saturation value of all the three pH values at a high oxygen level.

Introduction: The bloodstream absorbs the oxygen in the lungs in the human body, and from the lungs, transported to all the body tissues. Oxygen plays a vital role in the breathing processes and the metabolism processes of the living organisms.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
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Haematology - Red Blood Cell Life Cycle; Author: Armando Hasudungan;https://www.youtube.com/watch?v=cATQFej6oAc;License: Standard youtube license