How Sanger’s results support the results of the given experiment. Introduction: Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Question

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Answer 1

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?

Answer 2

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 3

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 6

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 7

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 8

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 9

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 10

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

Answer 11

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

Answer 12

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 13

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 14

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 15

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 16

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 17

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 18

Expert Solution & Answer

Answer 19

Expert Solution & Answer

Answer 20

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Check out a sample textbook solution

See solution

Answer 21

Ch. 3 - Prob. 1P Ch. 3 - Prob. 2P Ch. 3 - Prob. 3P Ch. 3 - Prob. 4P Ch. 3 - Prob. 5P Ch. 3 - Prob. 6P Ch. 3 - Prob. 7P Ch. 3 - Prob. 8P Ch. 3 - Prob. 9P Ch. 3 - Prob. 10P

Concept explainers

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Want to see the full answer?

Chapter 3 Solutions

Concept explainers

To explain: Whether results match the known sequence of bovine insulin. Introduction: Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin. Introduction: Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To explain: How the amino acid sequence of A chain was determined. Introduction: Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Want to see the full answer?

Chapter 3 Solutions

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.