How Sanger’s results support the results of the given experiment. Introduction: Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

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Answer 1

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

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You were tasked with purifying an untagged transcription factor (molecular weight 65,000Da, isoelectric point unknown) from a contaminant Protein A (molecular weight 50,000 Da, isoelectric point 5.0). You were also instructed to use the protein for crystallization studies after purification.i) In the initial purification step, which type of chromatography should you attempt? Explain your choice and specify the requirements of the buffer solution you should use. ii) Analyzing the proteins recovered from step i) using SDS-PAGE, you still observed a faint band of Protein A in addition to the transcription factor band. Given the limited time available for further purification, you must choose ONE appropriate chromatography method to maximize the chances of separating the two proteins as well as your crystallization studies. Provide a detailed explanation of your selection and the techniques/strategies you would employ to achieve this.

You were given a mixture of two proteins with different isoelectric points and molecular weights:• Protein X: pI 4.2, MW 42,000• Protein Y: pI 9.8, MW 90,000Using a Tris-glycine discontinuous native gel (pH8.3) electrophoresis system with a running gel of 12%, only a single band was observed upon protein staining after electrophoresis. Explain the observed result and discuss possible factors affecting protein migration in this system.

The standard cost of Product B manufactured by Oriole Company includes 3.5 units of direct materials at $5.40 per unit. During June, 27,300 units of direct materials are purchased at a cost of $5.15 per unit, and 27,300 units of direct materials are used to produce 7,600 units of Product B. (a) Compute the total materials variance and the price and quantity variances. Total materials variance Materials price variance Materials quantity variance $ (b) Compute the total materials variance and the price and quantity variances, assuming the purchase price is $6.35 and the quantity purchased and used is 26,300 units. Total materials variance Materials price variance Materials quantity variance $

Answer 2

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Expert Solution & Answer

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Answer 3

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 6

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 7

Chapter 3, Problem 23DAP

(a)

Summary Introduction

To explain: How Sanger’s results support the results of the given experiment.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 8

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 9

(b)

Summary Introduction

To check: Whether the results for the known structure of bovine insulin is consistent or not.

Introduction:

Proteins are composed of various amino acids residues through polymerization. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 10

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

Answer 11

(c)

Summary Introduction

To explain: The first four amino acids of the B chain based on the given data.

Introduction:

Proteins are the polymers of amino acids. These are basically macromolecules formed from polymerization of many amino acids. Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Given:

B1: α-DNP-phenylalanine only

B2: α-DNP-phenylalanine; valine

B3: aspartic acid; α-DNP-phenylalanine; valine

B4: aspartic acid; glutamic acid; α-DNP-phenylalanine; valine

Answer 12

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 13

(d1)

Summary Introduction

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 14

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 15

(d2)

Summary Introduction

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 16

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 17

(e)

Summary Introduction

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Answer 18

Expert Solution & Answer

Answer 19

Expert Solution & Answer

Answer 20

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Answer 21

Ch. 3 - Prob. 1P Ch. 3 - Prob. 2P Ch. 3 - Prob. 3P Ch. 3 - Prob. 4P Ch. 3 - Prob. 5P Ch. 3 - Prob. 6P Ch. 3 - Prob. 7P Ch. 3 - Prob. 8P Ch. 3 - Prob. 9P Ch. 3 - Prob. 10P

Concept explainers

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Want to see the full answer?

Chapter 3 Solutions

Concept explainers

To explain: Whether results match the known sequence of bovine insulin. Introduction: Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin. Introduction: Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To explain: How the amino acid sequence of A chain was determined. Introduction: Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

Want to see the full answer?

Chapter 3 Solutions

To explain: Whether results match the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To determine: If there are any discrepancies in the results when matched with the known sequence of bovine insulin.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.

To explain: How the amino acid sequence of A chain was determined.

Introduction:

Protein sequencing is a method to determine the amino acid sequence of a protein. Sanger used Sanger’s reagent in order to determine the structure of bovine insulin.