Concept explainers
Videos
Why do you expect colonies on the ampicillin agar to fluoresce?
To determine:
The reason for observation of the green fluorescent on ampicillin agar plate.
Introduction:
The gfp or the green flouroscent protein is a 238 amino acid long amino acid first isolated from Aequorea Victoria (avGFP). The GFP gives aa green fluorescent in visible light and blue fluorescent in UV. The GFP is very important for the microbial study as it allows tagging of protein and associated products and genes.
Explanation of Solution
In case, the ampicillin resistant genes is labelled as fluorescent, then on ampicillin rich agar, this gene will be expressed. The expression of the ampicillin resistance gene will cause the gfp protein to be expressed as a result green fluorescent will be observed.
Want to see more full solutions like this?
Chapter 29 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
- magine that you are conducting research on fruit type and seed dispersal. You submitted a paper to a peer-reviewed journal that addresses the factors that impact fruit type and seed dispersal mechanisms in plants of Central America. The editor of the journal communicates that your paper may be published if you make ‘minor revisions’ to the document. Describe two characteristics that you would expect in seeds that are dispersed by the wind. Contrast this with what you would expect for seeds that are gathered, buried or eaten by animals, and explain why they are different. (Editor’s note: Providing this information in your discussion will help readers to consider the significance of the research).arrow_forwardWhat is the difference between Uniporters, Symporters and Antiporters? Which of these are examples of active transport?arrow_forwardWhat are coupled transporters?arrow_forward
- How do histamine and prostaglandins help in the mobilization of leukocytes to an injury site? What are chemotactic factors? How do they affect inflammation process?arrow_forwardCompare and contrast neutrophils and macrophages. Describe two ways they are different and two ways they are similar.arrow_forwardDescribe the effects of three cytokines (not involved in the initial inflammation response). What cells release them?arrow_forward
