Biology
12th Edition
ISBN: 9781260494570
Author: Raven, Peter
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 29, Problem 2A
How could a plant without roots obtain sufficient nutrients from the soil?
a. It cannot: all land plants have roots.
b. Mycorrhizal
c. Charophytes associate with the plant and assist with the transfer of nutrients
d. It relies on its xylem in the absence of a root.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 29 Solutions
Biology
Ch. 29.1 - Explain the relationship between the different...Ch. 29.1 - Prob. 2LOCh. 29.1 - Prob. 3LOCh. 29.1 - Prob. 4LOCh. 29.2 - Describe adaptations of bryophytes for life on...Ch. 29.3 - Prob. 1LOCh. 29.3 - Evalute the signification of roots, stems, and...Ch. 29.4 - Prob. 1LOCh. 29.4 - Prob. 2LOCh. 29.5 - Prob. 1LO
Ch. 29.5 - Prob. 2LOCh. 29 - Compare the amount of time that passed between the...Ch. 29 - Explain why tracheophytes may have had a selective...Ch. 29 - Which of the following plant structures is NOT...Ch. 29 - Which of the following genera most likely directly...Ch. 29 - Which of the following would NOT be found in a...Ch. 29 - Prob. 4UCh. 29 - Evolutionary innovations that increase desiccation...Ch. 29 - Which of the following statements about the...Ch. 29 - Compare what happens to a spore mother cell as it...Ch. 29 - How could a plant without roots obtain sufficient...Ch. 29 - A major innovation of land plants is embryo...Ch. 29 - In comparing the Selaginella and Physcomitrella...Ch. 29 - Prob. 5ACh. 29 - Identify which of the following statements is...Ch. 29 - You have access to the sequenced genomes for moss...Ch. 29 - Would you expect the sporophyte generation of a...Ch. 29 - Imagine hypothetical moss and fern trees, each 10...
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