SAPLINGPLUS FOR PRINCIPLES OF BIOCHEMIS
SAPLINGPLUS FOR PRINCIPLES OF BIOCHEMIS
7th Edition
ISBN: 9781319424572
Author: nelson
Publisher: MAC HIGHER
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Chapter 28, Problem 5P
Summary Introduction

To calculate: The cellular concentration of the repressor protein.

Introduction: A repressor is a DNA binding or RNA binding molecule that binds with operator to inhibit the gene expression. DNA binding repressor inhibits the transcription as it block the promoter and prevent RNApolymerase attachment.

Summary Introduction

To compare: The cellular concentration of repressor protein with dissociation constant of the repressor-operator complex.

Introduction: The required number of repressor molecule to fill the half of operator site is defined by dissociation constant. The numbers of active repressor in the cell are the number of repressor molecule which always bound to operator site.

Summary Introduction

To explain: The significance of the answer in given data “repressor concentration E. coli”.

Introduction: The dissociation constant defines the required number of repressor molecule to fill the half of the operator site. The numbers of active repressor in the cell are the number of repressor molecule which always bound to operator site.

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1. Estimate the concentration of an enzyme within a living cell. Assume that:  (a): fresh tissue is 80% water and all of it is intracellular (b): the total soluble protein represents 15% of the weight (c): all the soluble proteins are enzymes (d): the average molecular weight of the proteins is 150,000 (E): about 100 different enzymes are present   please help I am lost
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