BIOLOGY
5th Edition
ISBN: 9781265202859
Author: BROOKER
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Chapter 27.3, Problem 1CC
Summary Introduction
To determine: The way through which endospores affect the disease causing abilities of certain bacteria.
Introduction: Endospores are resistant spores that are produced by bacterial cells by enclosure of DNA (Deoxyribose
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 27 Solutions
BIOLOGY
Ch. 27.2 - Core Skill: Connections Look ahead to Section...Ch. 27.2 - Prob. 2CSCh. 27.2 - Structure and Movement Concept Check: Does the...Ch. 27.2 - Prob. 3CSCh. 27.3 - Prob. 1CCCh. 27.5 - Prob. 1EQCh. 27.5 - Prob. 2EQCh. 27 - Which of the following features is common to...Ch. 27 - The bacterial phylum whose members typically...Ch. 27 - Gram-staining is a procedure that microbiologists...
Ch. 27 - Place the following steps in the correct order,...Ch. 27 - What ecological role do cyanobacteria play? a....Ch. 27 - Prob. 6TYCh. 27 - Prob. 7TYCh. 27 - Prob. 8TYCh. 27 - Prob. 9TYCh. 27 - Prob. 10TYCh. 27 - Prob. 1CQCh. 27 - Prob. 2CQCh. 27 - Prob. 3CQCh. 27 - Prob. 1COQCh. 27 - Prob. 2COQ
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- is often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forward
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